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    biotin-follistatin-antibody-ab47942.pdf

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Signal Transduction Growth Factors/Hormones Hormones
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Biotin Anti-Follistatin antibody (ab47942)

  • Datasheet
Submit a review Submit a question References (1)

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Western blot - Biotin Anti-Follistatin antibody (ab47942)
  • Sandwich ELISA - Biotin Anti-Follistatin antibody (ab47942)

Key features and details

  • Biotin Rabbit polyclonal to Follistatin
  • Suitable for: Sandwich ELISA, WB
  • Reacts with: Human
  • Conjugation: Biotin
  • Isotype: IgG

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Recombinant human Follistatin protein (ab50163)

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Overview

  • Product name

    Biotin Anti-Follistatin antibody
    See all Follistatin primary antibodies
  • Description

    Biotin Rabbit polyclonal to Follistatin
  • Host species

    Rabbit
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: Sandwich ELISA, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length Follistatin protein (Human) highly pure (>98%)

  • Positive control

    • Purchase matching WB positive control:Recombinant Human Follistatin protein
    • WB: Recombinant human Follistatin protein (ab50163)
  • General notes

    Once frozen, avoid more than one freeze-thaw cycle.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Lyophilized:Reconstitute with 1ml sterile PBS solution containing 0.1% BSA.
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was purified by affinity chromatography and then biotinylated.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • Other

Associated products

  • Isotype control

    • Biotin Rabbit IgG - Isotype Control (ab200208)
  • Recombinant Protein

    • Recombinant human Follistatin protein (ab50163)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab47942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
Use a concentration of 0.25 - 1 µg/ml.

This allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human Follistatin using 100 µl/well.

WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 38 kDa.
Notes
Sandwich ELISA
Use a concentration of 0.25 - 1 µg/ml.

This allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human Follistatin using 100 µl/well.

WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 38 kDa.

Target

  • Function

    Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH).
  • Tissue specificity

    Isoform 1 is the predominant isoform in serum but is undetectable in follicular fluid.
  • Sequence similarities

    Contains 3 follistatin-like domains.
    Contains 3 Kazal-like domains.
    Contains 1 TB (TGF-beta binding) domain.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P19883 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10468 Human
    • Omim: 136470 Human
    • SwissProt: P19883 Human
    • Unigene: 9914 Human
    • Alternative names

      • Activin binding protein antibody
      • Activin-binding protein antibody
      • Follistatin antibody
      • FS antibody
      • fst antibody
      • FST_HUMAN antibody
      • Human follistatin gene antibody
      see all

    Images

    • Western blot - Biotin Anti-Follistatin antibody (ab47942)
      Western blot - Biotin Anti-Follistatin antibody (ab47942)

      All lanes: Anti-Follistatin antibody (Biotin) (ab47942) at 0.2 µg/ml

       

      Lane 1: Marker

       

      Unreduced Recombinant human Follistatin protein (ab50163):

      Lane 2: 250 ng

      Lane 3: 125 ng

      Lane 4: 62.5 ng

      Lane 5: 31.25 ng

      Lane 6: 15.625 ng

      Lane 7: 7.8 ng

      Lane 8: 3.9 ng

      Lane 9: 1.95 ng

      Lane 10: 0.975 ng

      Lane 11: 0.4875 ng

      Lane 12: 0.24 ng

    • Sandwich ELISA - Biotin Anti-Follistatin antibody (ab47942)
      Sandwich ELISA - Biotin Anti-Follistatin antibody (ab47942)

      To detect Human Follistatin by sandwich ELISA (using 100 mL/well) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Rabbit polyclonal to Follistatin (ab47941) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human Follistatin.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (1)

    Publishing research using ab47942? Please let us know so that we can cite the reference in this datasheet.

    ab47942 has been referenced in 1 publication.

    • Walpurgis K  et al. Detection of follistatin-based inhibitors of the TGF-ß signaling pathways in serum/plasma by means of LC-HRMS/MS and Western blotting. Drug Test Anal 12:1636-1648 (2020). PubMed: 32959984

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