Biotin Anti-Follistatin antibody (ab47942)
Key features and details
- Biotin Rabbit polyclonal to Follistatin
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-Follistatin antibody
See all Follistatin primary antibodies -
Description
Biotin Rabbit polyclonal to Follistatin -
Host species
Rabbit -
Conjugation
Biotin -
Tested applications
Suitable for: Sandwich ELISA, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length Follistatin protein (Human) highly pure (>98%)
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Positive control
- Purchase matching WB positive control:Recombinant Human Follistatin protein
- WB: Recombinant human Follistatin protein (ab50163)
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General notes
Once frozen, avoid more than one freeze-thaw cycle.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute with 1ml sterile PBS solution containing 0.1% BSA. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was purified by affinity chromatography and then biotinylated. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA |
Use a concentration of 0.25 - 1 µg/ml.
This allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human Follistatin using 100 µl/well. |
|
| WB |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 38 kDa.
|
| Notes |
|---|
|
Sandwich ELISA
Use a concentration of 0.25 - 1 µg/ml. This allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human Follistatin using 100 µl/well. |
|
WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 38 kDa. |
Target
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Function
Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH). -
Tissue specificity
Isoform 1 is the predominant isoform in serum but is undetectable in follicular fluid. -
Sequence similarities
Contains 3 follistatin-like domains.
Contains 3 Kazal-like domains.
Contains 1 TB (TGF-beta binding) domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 10468 Human
- Omim: 136470 Human
- SwissProt: P19883 Human
- Unigene: 9914 Human
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Alternative names
- Activin binding protein antibody
- Activin-binding protein antibody
- Follistatin antibody
see all
Images
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Western blot - Biotin Anti-Follistatin antibody (ab47942)
All lanes: Anti-Follistatin antibody (Biotin) (ab47942) at 0.2 µg/ml
Lane 1: Marker
Unreduced Recombinant human Follistatin protein (ab50163):
Lane 2: 250 ng
Lane 3: 125 ng
Lane 4: 62.5 ng
Lane 5: 31.25 ng
Lane 6: 15.625 ng
Lane 7: 7.8 ng
Lane 8: 3.9 ng
Lane 9: 1.95 ng
Lane 10: 0.975 ng
Lane 11: 0.4875 ng
Lane 12: 0.24 ng
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Sandwich ELISA - Biotin Anti-Follistatin antibody (ab47942)To detect Human Follistatin by sandwich ELISA (using 100 mL/well) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Rabbit polyclonal to Follistatin (ab47941) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human Follistatin.
Datasheets and documents
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Datasheet download
References (1)
ab47942 has been referenced in 1 publication.
- Walpurgis K et al. Detection of follistatin-based inhibitors of the TGF-ß signaling pathways in serum/plasma by means of LC-HRMS/MS and Western blotting. Drug Test Anal 12:1636-1648 (2020). PubMed: 32959984
