Biotin Anti-Follistatin antibody (ab47942)
Key features and details
- Biotin Rabbit polyclonal to Follistatin
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-Follistatin antibody
See all Follistatin primary antibodies -
Description
Biotin Rabbit polyclonal to Follistatin -
Host species
Rabbit -
Conjugation
Biotin -
Tested applications
Suitable for: Sandwich ELISA, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length Follistatin protein (Human) highly pure (>98%)
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Positive control
- Purchase matching WB positive control:Recombinant Human Follistatin protein
- WB: Recombinant human Follistatin protein (ab50163)
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General notes
Once frozen, avoid more than one freeze-thaw cycle.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Lyophilized:Reconstitute with 1ml sterile PBS solution containing 0.1% BSA. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was purified by affinity chromatography and then biotinylated. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab47942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use a concentration of 0.25 - 1 µg/ml. This allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human Follistatin using 100 µl/well. |
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| WB | Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 38 kDa. |
Target
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Function
Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH). -
Tissue specificity
Isoform 1 is the predominant isoform in serum but is undetectable in follicular fluid. -
Sequence similarities
Contains 3 follistatin-like domains.
Contains 3 Kazal-like domains.
Contains 1 TB (TGF-beta binding) domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 10468 Human
- Omim: 136470 Human
- SwissProt: P19883 Human
- Unigene: 9914 Human
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Alternative names
- Activin binding protein antibody
- Activin-binding protein antibody
- Follistatin antibody
see all
Images
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Western blot - Biotin Anti-Follistatin antibody (ab47942)
All lanes: Anti-Follistatin antibody (Biotin) (ab47942) at 0.2 µg/ml
Lane 1: Marker
Unreduced Recombinant human Follistatin protein (ab50163):
Lane 2: 250 ng
Lane 3: 125 ng
Lane 4: 62.5 ng
Lane 5: 31.25 ng
Lane 6: 15.625 ng
Lane 7: 7.8 ng
Lane 8: 3.9 ng
Lane 9: 1.95 ng
Lane 10: 0.975 ng
Lane 11: 0.4875 ng
Lane 12: 0.24 ng
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Sandwich ELISA - Biotin Anti-Follistatin antibody (ab47942)To detect Human Follistatin by sandwich ELISA (using 100 mL/well) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Rabbit polyclonal to Follistatin (ab47941) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human Follistatin.
Datasheets and documents
References (0)
ab47942 has not yet been referenced specifically in any publications.
