Key features and details
- Biotin Rabbit polyclonal to Follistatin
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Product nameBiotin Anti-Follistatin antibody
See all Follistatin primary antibodies
DescriptionBiotin Rabbit polyclonal to Follistatin
Tested applicationsSuitable for: Sandwich ELISA, WBmore details
Species reactivityReacts with: Human
Recombinant full length Follistatin protein (Human) highly pure (>98%)
- Purchase matching WB positive control:Recombinant Human Follistatin protein
- WB: Recombinant human Follistatin protein (ab50163)
General notesOnce frozen, avoid more than one freeze-thaw cycle.
FormLyophilized:Reconstitute with 1ml sterile PBS solution containing 0.1% BSA.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituents: PBS, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was purified by affinity chromatography and then biotinylated.
Our Abpromise guarantee covers the use of ab47942 in the following tested applications.
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml.
This allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human Follistatin using 100 µl/well.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 38 kDa.|
FunctionBinds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH).
Tissue specificityIsoform 1 is the predominant isoform in serum but is undetectable in follicular fluid.
Sequence similaritiesContains 3 follistatin-like domains.
Contains 3 Kazal-like domains.
Contains 1 TB (TGF-beta binding) domain.
- Information by UniProt
- Activin binding protein antibody
- Activin-binding protein antibody
- Follistatin antibody
All lanes: Anti-Follistatin antibody (Biotin) (ab47942) at 0.2 µg/ml
Lane 1: Marker
Unreduced Recombinant human Follistatin protein (ab50163):
Lane 2: 250 ng
Lane 3: 125 ng
Lane 4: 62.5 ng
Lane 5: 31.25 ng
Lane 6: 15.625 ng
Lane 7: 7.8 ng
Lane 8: 3.9 ng
Lane 9: 1.95 ng
Lane 10: 0.975 ng
Lane 11: 0.4875 ng
Lane 12: 0.24 ng
To detect Human Follistatin by sandwich ELISA (using 100 mL/well) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Rabbit polyclonal to Follistatin (ab47941) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human Follistatin.
ab47942 has not yet been referenced specifically in any publications.