Biotin Anti-Galectin 3 antibody [M3/38] (ab95679)
![Flow Cytometry - Biotin Anti-Galectin 3 antibody [M3/38] (ab95679)](https://www.abcam.com/ps/products/95/ab95679/Images/Galectin-3-Primary-antibodies-ab95679-1.gif "Flow Cytometry - Biotin Anti-Galectin 3 antibody [M3/38] (ab95679)")
Key features and details
- Biotin Rat monoclonal [M3/38] to Galectin 3
- Suitable for: Flow Cyt
- Reacts with: Mouse, Human
- Conjugation: Biotin
- Isotype: IgG2a
Overview
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Product name
Biotin Anti-Galectin 3 antibody [M3/38]
See all Galectin 3 primary antibodies -
Description
Biotin Rat monoclonal [M3/38] to Galectin 3 -
Host species
Rat -
Conjugation
Biotin -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Galectin 3 of mouse origin
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Positive control
- Normal human peripheral blood cells, C57BL/6 Mouse bone marrow cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
M3/38 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab95679 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 0.25µg for 105 cells. ab95679 can be used at 0.25 µg/per test. A test is defined as the amount (µg)/test of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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Target
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Function
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. -
Tissue specificity
A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. -
Sequence similarities
Contains 1 galectin domain. -
Cellular localization
Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface. - Information by UniProt
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Database links
- Entrez Gene: 3958 Human
- Entrez Gene: 16854 Mouse
- Omim: 153619 Human
- SwissProt: P17931 Human
- SwissProt: P16110 Mouse
- Unigene: 531081 Human
- Unigene: 248615 Mouse
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Alternative names
- 35 kDa lectin antibody
- Carbohydrate binding protein 35 antibody
- Carbohydrate-binding protein 35 antibody
see all
Images
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Flow Cytometry - Biotin Anti-Galectin 3 antibody [M3/38] (ab95679)ab95679 at 0.125µg dilution staining Galectin 3 in C57BL/6 mouse bone marrow cells by Flow Cytometry (shown in blue) followed by Streptavidin PE.
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![Flow Cytometry - Biotin Anti-Galectin 3 antibody [M3/38] (ab95679)](https://www.abcam.com/ps/products/95/ab95679/Images/Galectin-3-Primary-antibodies-ab95679-2.gif)
Flow Cytometry - Biotin Anti-Galectin 3 antibody [M3/38] (ab95679)ab95679 at 0.125µg dilution staining the monocyte population in normal human peripheral blood cells by Flow Cytometry followed by Streptavidin PE.
Datasheets and documents
References (0)
ab95679 has not yet been referenced specifically in any publications.
