Recombinant Biotin Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab197629)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab197629)](https://www.abcam.com/ps/products/197/ab197629/Images/ab197629-238409-IHCAP22255831.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab197629)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Biotin Rabbit monoclonal [EPR13872] to gamma Tubulin - Centrosome Marker
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: Biotin
Related conjugates and formulations
Overview
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Product name
Biotin Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker
See all gamma Tubulin primary antibodies -
Description
Biotin Rabbit monoclonal [EPR13872] to gamma Tubulin - Centrosome Marker -
Host species
Rabbit -
Conjugation
Biotin -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Drosophila melanogaster, a wide range of other species
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Normal human colon tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13872 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab197629 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation. -
Sequence similarities
Belongs to the tubulin family. -
Post-translational
modificationsPhosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome. -
Cellular localization
Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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Database links
- Entrez Gene: 540216 Cow
- Entrez Gene: 541101 Cow
- Entrez Gene: 403695 Dog
- Entrez Gene: 490962 Dog
- Entrez Gene: 35199 Drosophila melanogaster
- Entrez Gene: 27175 Human
- Entrez Gene: 7283 Human
- Entrez Gene: 103733 Mouse
see all -
Alternative names
- Gamma 1 tubulin antibody
- Gamma 2 tubulin antibody
- Gamma Tubulin 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab197629)
IHC image of gamma Tubulin staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab197629 at 1/500 dilution for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Biotin Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab197629)](https://www.abcam.com/ps/products/197/ab197629/Images/ab197629-2-benefits-of-recombinant-antibodies.png)
Biotin Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab197629)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab197629 has been referenced in 1 publication.
- Myung Park J et al. Beclin 1 and UVRAG Confer Protection from Radiation-Induced DNA Damage and Maintain Centrosome Stability in Colorectal Cancer Cells. PLoS One 9:e100819 (2014). WB . PubMed: 24956373