Biotin Anti-Ghrelin antibody (ab48285)

Submit a review Q&A (5)

Key features and details

  • Biotin Rabbit polyclonal to Ghrelin
  • Suitable for: ELISA, IP, IHC-P, RIA
  • Reacts with: Human
  • Conjugation: Biotin
  • Isotype: IgG

Overview

  • Product name

    Biotin Anti-Ghrelin antibody
    See all Ghrelin primary antibodies

  • Description

    Biotin Rabbit polyclonal to Ghrelin

  • Host species

    Rabbit

  • Conjugation

    Biotin

  • Tested applications

    Suitable for: ELISA, IP, IHC-P, RIAmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cat, Dog, Pig, Baboon, Rhesus monkey, Gerbil

  • Immunogen

    Synthetic peptide corresponding to Human Ghrelin aa 1-100 (internal sequence) conjugated to keyhole limpet haemocyanin.
    Database link: Q9UBU3-1
    (Peptide available as ab89233)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab48285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
RIA
Use at an assay dependent concentration.
Notes
ELISA
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
RIA
Use at an assay dependent concentration.

Target

  • Function

    Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.
    Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility.

  • Tissue specificity

    Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.

  • Sequence similarities

    Belongs to the motilin family.

  • Post-translational
    modifications

    O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid.
    Amidation of Leu-98 is essential for obestatin activity.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Appetite regulating hormone antibody
    • Ghrelin 27 antibody
    • Ghrelin 28 antibody
    • Ghrelin and obestatin prepropeptide antibody
    • Ghrelin antibody
    • Ghrelin/obestatin prepropeptide antibody
    • ghrl antibody
    • GHRL_HUMAN antibody
    • Growth hormone releasing peptide antibody
    • Growth hormone secretagogue antibody
    • Growth hormone-releasing peptide antibody
    • M46 protein antibody
    • Motilin related peptide antibody
    • Motilin-related peptide antibody
    • MTLRP antibody
    • Obestatin antibody
    • Protein M46 antibody
    see all

References (0)

Publishing research using ab48285? Please let us know so that we can cite the reference in this datasheet.

ab48285 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-5 of 5 Abreviews or Q&A

Question

The antibody ab48285 at 4 ug/ml did not work well in our system. In fact, by using this much antibody, we can only make 25 ml of 4 ug/ml. In each RIA tube, we have the incubation volume of 0.5 ml. Thus, with 100 ug of ab48285 we can only run 50 tubes. If we would run ab48285 at 8 ug/ml, then we can only run 25 tubes. As you can see that this would not be economical. When we ordered this antibody, we were hoping that it would work out at 2 ug/ml; we tested both 2 ug/ml and 4 ug/ml, they yielded 5% and 10 % specific binding, respectively. With this kind of binding result, I do not expect 8ug/ml of ab 48285 will give us adequate binding for RIA.

I hope you will give us credit at this time.

We had good experience with your products, and will not hesitate to try more of your products in the future.

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Answer

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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Question

I was testing your product rabbit polyclonal to ghrelin (ab48285) in our RIA system, and it failed. Following your instruction, I used 4 ug/ml but the specific binding of 125I-ghrelin was only 10%, whereas the antibody from another company showed 30% specific binding. I understand that you would refund the cost if the customer is not satisfied with your product.

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Answer

Thank you for contacting Abcam regarding ab48285.


I am sorry that you have been experiencing difficulties with this antibody in your RIA experiments. I understand that you have tested a concentration of 4ug/ml - have you tested any others? We recommend up to 8ug/ml. Some further optimization may be required for best results with this antibody.


If increasing the amount of antibody used does not improve your results with this antibody, I am happy to offer a replacement or credit. Please do not hesitate to contact me if you have any additional questions.

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Question

I am interested in using your product ab48285 to set up an RIA of ghrelin. Do you have any information regarding the use of this product for RIA? For example, I would like to know how much I should dilute this antibody to do RIA? What is the lowest concentration I can detect using this antibody?

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Answer

Thank you for contacting us.

The supplying lab sent us the following information:

The antibody isrecommended from 2-8 ug/ml for RIA purpose. The efficiency of labeling of their tracer is another factor to determine the concentration use for their RIA assay.The optimal condition would need to be determined by the enduser.

I am sorry that we did not receive more detailed information than this.
Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
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Question

Greetings- I would like to kindly ask you to help me select the  appropriate Elisa tests for the following products:   immunoglobulin G and A autoantibodies against ghrelin, orexin, leptin, insulin, neuropeptide Y,  peptide YY, ACTH and  alfa MCH.   Thank you in advance for your help. I really appreciate it.   Best regards,

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Answer

Thank you for contacting us. Please choose antibodies from the following catalogue numbers: anti Ghrelin ab48285, ab117620, ab15861, ab57222, ab104307 These antibodies are tested in Indirect ELISA so are guaranteed for this application and applications as stated on the datasheets. Anti Orexin ab35337, ab6214, ab16787, ab64565 These antibodies are tested in Indirect ELISA so are guaranteed for this application and applications as stated on the datasheets. Anti leptin ab20101, ab20115, ab9749 and ab2095 Anti Insulin ab6995, ab63820, ab7760 and ab46707 Anti neuropeptide Y We ab30914, ab10341, ab6173, ab43871, ab53478. These antibodies are not yet tested in ELISA. Anti peptide YY  ab51537, ab51539 etc. Anti ACTH  ab20358, ab21003, ab104768,a b104619 Anti MCH ab37337; These are however not yet tested in ELISA. Please check the datasheets of these antibodies for more up-to-date information. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Is this antibody specific for octanoyl-ated version of protein?

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Answer

ab48285 has not been tested to determine if the antibody will bind Ghrelin whether it has octanoyl or no octanoyl.  Sorry this aspect of ab48285 has not yet been charactarized. If you have any other questions please feel free to contact me.

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