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Cardiovascular Blood Blood Cell Antigens RBC Antigens
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Biotin Anti-Glycophorin A + B antibody [HIR2] (ab93548)

  • Datasheet
Reviews (1)Q&A (1)References (1)

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Key features and details

  • Biotin Mouse monoclonal [HIR2] to Glycophorin A + B
  • Reacts with: Human
  • Conjugation: Biotin
  • Isotype: IgG2b

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Overview

  • Product name

    Biotin Anti-Glycophorin A + B antibody [HIR2]
    See all Glycophorin A + B primary antibodies
  • Description

    Biotin Mouse monoclonal [HIR2] to Glycophorin A + B
  • Host species

    Mouse
  • Conjugation

    Biotin
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Glycophorin A + B (N terminal).

  • Positive control

    • Human peripheral blood leukocytes
  • General notes

    Mouse monoclonal [HIR2] to Glycophorin A (Biotin) also has a clone number GA-R2.


    Binding of ab93548 to red cells at high antibody concentration causes cell agglutination.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: PBS, 0.1% Gelatin
  • Concentration information loading...
  • Purity

    Protein G purified
  • Primary antibody notes

    Binding of ab93548 to red cells at high antibody concentration causes cell agglutination.
  • Clonality

    Monoclonal
  • Clone number

    HIR2
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • RBC Antigens
    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • RBC Antigens
    • Blood Group Antigens

Associated products

  • Alternative Versions

    • Anti-Glycophorin A + B antibody [HIR2] (ab15009)
    • PE Anti-Glycophorin A + B antibody [HIR2] (ab26016)
    • FITC Anti-Glycophorin A + B antibody [HIR2] (ab52452)
  • Isotype control

    • Biotin Mouse IgG2b, kappa monoclonal [MPC-11] - Isotype Control (ab18418)

Target

  • Relevance

    Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. GYPA gene consists of 7 exons and has 97% sequence homology with GYPB from the 5' UTR to the coding sequence encoding the first 45 amino acids. GYPB accounts for S, s and U specificities. GPA and GPB provide the cells with a large mucin-like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta; also, Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are resulted from gene recombinations between GYPA and GYPB. These antigens are expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative).
  • Cellular localization

    Type I membrane protein.
  • Database links

    • Entrez Gene: 2993 Human
    • Entrez Gene: 2994 Human
    • SwissProt: P02724 Human
    • SwissProt: P06028 Human
    • Unigene: 434973 Human
    • Unigene: 654368 Human
    • Alternative names

      • CD235a antibody
      • CD235b antibody
      • Erythroid lineage specific membrane sialoglycoprotein antibody
      • Glycophorin Erik antibody
      • Glycophorin HeP2 antibody
      • Glycophorin MiI antibody
      • Glycophorin MiIII antibody
      • Glycophorin MiV antibody
      • Glycophorin MiVI antibody
      • Glycophorin MiX antibody
      • Glycophorin SAT antibody
      • Glycophorin Sta type C antibody
      • GPA antibody
      • GPB antibody
      • GPB.NY antibody
      • GPErik antibody
      • GpMiIII antibody
      • GPSAT antibody
      • GYPA antibody
      • GYPB antibody
      • GYPHe.NY antibody
      • HGpMiIII antibody
      • HGpMiV antibody
      • HGpMiVI antibody
      • HGpMiX antibody
      • HGpMiXI antibody
      • HGpSta(C) antibody
      • Mi.V glycoprotein (24 AA) antibody
      • MN antibody
      • MN sialoglycoprotein antibody
      • MNS antibody
      • PAS 2 antibody
      • PAS 3 antibody
      • PAS2 antibody
      • PAS3 antibody
      • Sialoglycoprotein alpha antibody
      • Sialoglycoprotein delta antibody
      • SS active sialoglycoprotein antibody
      • SS antibody
      see all

    Protocols

    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab93548? Please let us know so that we can cite the reference in this datasheet.

    ab93548 has been referenced in 1 publication.

    • Chen G  et al. [Immunophenotyping study on the blood cells of the patients with paroxysmal nocturnal hemoglobinuria] Zhonghua Xue Ye Xue Za Zhi 18:190-2 (1997). PubMed: 15622765

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    I am presently using your product and would like to know the specs on the quantification of Moles of Biotin, per mole of the antibody please.
    Also, it is stated under "Primary antibody notes" Binding of ab93548 to red cells at high antibody concentration causes cell agglutination. What is considered a high antibody concentration?
    Thank you in advance,

    Read More

    Abcam community

    Verified customer

    Asked on Mar 29 2012

    Answer

    Thank you for contacting us with your questions, and for your patience while I have been in touch with the lab for more information about ab93548.

    The lab has informed me that there are approximately 3-7 molecules of biotin per antibody.The clone HIR2is known to causered blood cell agglutination however we have not tested this in-house and I could not find a specific concentration that was used.I've not been able to find any additional information in the literature about this close in agglutination assays.

    I apologize thatwe don't have this information, but please let me know if you have any additional question or if there is anything else that we can do for you.

    Read More

    Abcam Scientific Support

    Answered on Mar 29 2012

    Flow Cytometry abreview for Anti-Glycophorin A + B [HIR2] antibody (Biotin)

    Good
    Abreviews
    Abreviews
    Application
    Flow Cytometry
    Sample
    Human Cell (PBMC)
    Specification
    PBMC
    Permeabilization
    No
    Read More

    Dr. Elodie Segura

    Verified customer

    Submitted Jan 25 2011

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