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    biotin-grb2-antibody-y237-ab201539.pdf

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Signal Transduction Adapters Cytoplasmic
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RecombinantRabMAb

Recombinant Biotin Anti-GRB2 antibody [Y237] (ab201539)

  • Datasheet
  • Certificate of Compliance
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-GRB2 antibody [Y237] (ab201539)
  • Biotin Anti-GRB2 antibody [Y237] (ab201539)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Biotin Rabbit monoclonal [Y237] to GRB2
  • Suitable for: IHC-P
  • Reacts with: Human
  • Conjugation: Biotin

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Protein
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Recombinant Human GRB2 protein (ab116430)
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Streptavidin HRP (ready-to-use) (ab64269)

View more associated products

Overview

  • Product name

    Biotin Anti-GRB2 antibody [Y237]
    See all GRB2 primary antibodies
  • Description

    Biotin Rabbit monoclonal [Y237] to GRB2
  • Host species

    Rabbit
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human GRB2 aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control

    • IHC-P: normal human colon tissue sections
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    Y237
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Adapters
    • Cytoplasmic
    • Cancer
    • Signal transduction
    • G protein signaling
    • Small G proteins
    • Ras family

Associated products

  • Alternative Versions

    • HRP Anti-GRB2 antibody [Y237] (ab201865)
    • Anti-GRB2 antibody [Y237] - BSA and Azide free (ab227117)
    • Alexa Fluor® 488 Anti-GRB2 antibody [Y237] (ab252037)
    • Anti-GRB2 antibody [Y237] (ab32037)
  • Isotype control

    • Biotin Rabbit IgG - Isotype Control (ab200208)
  • Recombinant Protein

    • Recombinant Human GRB2 protein (ab116430)

Applications

Our Abpromise guarantee covers the use of ab201539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Target

  • Function

    Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
    Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
  • Sequence similarities

    Belongs to the GRB2/sem-5/DRK family.
    Contains 1 SH2 domain.
    Contains 2 SH3 domains.
  • Domain

    The SH3 domains mediate interaction with RALGPS1 and SHB.
  • Cellular localization

    Golgi apparatus.
  • Target information above from: UniProt accession P62993 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2885 Human
    • Entrez Gene: 14784 Mouse
    • Entrez Gene: 81504 Rat
    • Omim: 108355 Human
    • SwissProt: P62993 Human
    • SwissProt: Q60631 Mouse
    • SwissProt: P62994 Rat
    • Unigene: 444356 Human
    • Unigene: 708119 Human
    • Unigene: 439649 Mouse
    • Unigene: 3360 Rat
    see all
  • Alternative names

    • Abundant SRC homology antibody
    • Adapter protein GRB2 antibody
    • ASH antibody
    • Ash protein antibody
    • EGFRBP GRB2 antibody
    • Epidermal growth factor receptor binding protein antibody
    • Epidermal growth factor receptor binding protein GRB2 antibody
    • GRB 2 antibody
    • GRB2 adapter protein antibody
    • Grb2 antibody
    • GRB2_HUMAN antibody
    • Grb3 3 antibody
    • Growth factor receptor bound protein 2 antibody
    • Growth factor receptor bound protein 3 antibody
    • Growth factor receptor-bound protein 2 antibody
    • HT027 antibody
    • MST084 antibody
    • MSTP084 antibody
    • NCKAP2 antibody
    • OTTHUMP00000166096 antibody
    • OTTHUMP00000166097 antibody
    • OTTHUMP00000166098 antibody
    • Protein ash antibody
    • SEM5 antibody
    • SH2/SH3 adapter GRB2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-GRB2 antibody [Y237] (ab201539)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-GRB2 antibody [Y237] (ab201539)

    IHC image of GRB2 staining in a section of formalin-fixed paraffin-embedded normal human colon*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab201539 at 1/100 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Biotin Anti-GRB2 antibody [Y237] (ab201539)
    Biotin Anti-GRB2 antibody [Y237] (ab201539)

Protocols

  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (2)

    Publishing research using ab201539? Please let us know so that we can cite the reference in this datasheet.

    ab201539 has been referenced in 2 publications.

    • Chang W  et al. Gene expression profiling-derived immunohistochemistry signature with high prognostic value in colorectal carcinoma. Gut N/A:N/A (2013). WB ; Human . PubMed: 24173294
    • Moses J  et al. Intended transcriptional silencing with siRNA results in gene repression through sequence-specific off-targeting. RNA 16:430-41 (2010). WB ; Human . PubMed: 20026621

    Customer reviews and Q&As

    Show All Reviews Q&A
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    There are currently no Customer reviews or Questions for ab201539.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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