Biotin Anti-GST antibody (ab71283)

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ELISA - Biotin Anti-GST antibody (ab71283)

    Key features and details

    • Biotin Goat polyclonal to GST
    • Suitable for: ELISA, WB, IHC-P
    • Reacts with: Species independent
    • Conjugation: Biotin
    • Isotype: IgG

    Overview

    • Product name

      Biotin Anti-GST antibody
      See all GST primary antibodies

    • Description

      Biotin Goat polyclonal to GST

    • Host species

      Goat

    • Conjugation

      Biotin

    • Specificity

      Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum]. Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.

    • Tested applications

      Suitable for: ELISA, WB, IHC-Pmore details

    • Species reactivity

      Reacts with: Species independent

    • Immunogen

      Glutathione-S-Transferase [Schistosoma japonicum]

    • General notes

      The antibody was labelled with Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Associated products

    Applications

    Our Abpromise guarantee covers the use of ab71283 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA Use at an assay dependent concentration.
    WB Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
    IHC-P Use at an assay dependent concentration.

    Target

    • Relevance

      GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.

    • Alternative names

      • Glutathione S Transferase antibody
      • Glutathione S-transferase class-mu 26 kDa isozyme antibody
      • GST antibody
      • Sj26 antigen antibody
      • SjGST antibody
      see all

    Images

    • ELISA - Biotin Anti-GST antibody (ab71283)
      ELISA - Biotin Anti-GST antibody (ab71283)
      ELISA results of ab71283 tested against purified GST. Each well was coated in duplicate with 1.0 µg of GST. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Streptavidin Peroxidase Conjugated and TMB substrate.

    References (1)

    Publishing research using ab71283? Please let us know so that we can cite the reference in this datasheet.

    ab71283 has been referenced in 1 publication.

    • Rockel S  et al. iSLIM: a comprehensive approach to mapping and characterizing gene regulatory networks. Nucleic Acids Res 41:e52 (2013). PubMed: 23258699

    Customer reviews and Q&As

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    Question

    Would like to try this antibody with a magnetic bead isolation assay.

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    Answer

    Thank you for your call today and for your questions.

    Since ab71283 has not been tested in this kind of assay,I can offer a discount off a future purchase if you buy ab71283 now, test it in your assay and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would beworth 1 free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test the antibody. I will then send a discount code.

    2. Purchase ab71283 either by phone, fax, or online (www.abcam.com).

    3. Test it in your assay.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. I would recommend using our form for immunoprecipitation (IP) since this seems to be the most similar to your isolation assay.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.
    Please remember that submission of the Abreview is sufficient for the discount code to become active.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab71283 turns out to be unsuitable, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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