Biotin Anti-GSTA1 antibody [8-326] (ab173835)

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Western blot - Biotin Anti-GSTA1 antibody [8-326] (ab173835)

    Key features and details

    • Biotin Mouse monoclonal [8-326] to GSTA1
    • Suitable for: WB
    • Reacts with: Human
    • Conjugation: Biotin
    • Isotype: IgG

    Overview

    • Product name

      Biotin Anti-GSTA1 antibody [8-326]
      See all GSTA1 primary antibodies

    • Description

      Biotin Mouse monoclonal [8-326] to GSTA1

    • Host species

      Mouse

    • Conjugation

      Biotin

    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data

    • Immunogen

      Recombinant full length protein corresponding to Human GSTA1 aa 1-222.
      Sequence:

      MAEKPKLHYFNARGRMESTRWLLAAAGVEFEEKFIKSAEDLDKLRNDGYL MFQQVPMVEIDGMKLVQTRAILNYIASKYNLYGKDIKERALIDMYIEGIA DLGEMILLLPVCPPEEKDAKLALIKEKIKNRYFPAFEKVLKSHGQDYLVG NKLSRADIHLVELLYYVEELDSSLISSFPLLKALKTRISNLPTVKKFLQP GSPRKPPMDEKSLEEARKIFRF


      Database link: P08263
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • Purified Glutathione S Transferase-SRC SH2 domain

    • General notes

      Previously labelled as Glutathione S Transferase alpha 1. 

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

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    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab173835 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB
    1/500 - 1/1000. Predicted molecular weight: 26 kDa.
    Notes
    WB
    1/500 - 1/1000. Predicted molecular weight: 26 kDa.

    Target

    • Function

      Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

    • Tissue specificity

      Liver.

    • Sequence similarities

      Belongs to the GST superfamily. Alpha family.
      Contains 1 GST C-terminal domain.
      Contains 1 GST N-terminal domain.

    • Domain

      The C-terminal domain may form a component of the hydrophobic substrate-binding site, but in contrast appears not to be directly involved in GSH binding and is not absolutely essential for catalytic activity.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      • Alternative names

        • Glutathione S alkyltransferase A1 antibody
        • Glutathione S aryltransferase A1 antibody
        • Glutathione S transferase 2 antibody
        • Glutathione S transferase A1 antibody
        • Glutathione S transferase alpha 1 antibody
        • Glutathione S transferase Ha subunit 1 antibody
        • Glutathione S-transferase A1 antibody
        • GST class-alpha member 1 antibody
        • GST epsilon antibody
        • GST HA subunit 1 antibody
        • GST, class alpha, 1 antibody
        • GST-epsilon antibody
        • GST2 antibody
        • GSTA1 1 antibody
        • GSTA1 antibody
        • GSTA1-1 antibody
        • GSTA1_HUMAN antibody
        • GTH1 antibody
        • HA subunit 1 antibody
        • MGC131939 antibody
        • OTTHUMP00000016611 antibody
        • S (hydroxyalkyl)glutathione lyase A1 antibody
        see all

      Images

      • Western blot - Biotin Anti-GSTA1 antibody [8-326] (ab173835)
        Western blot - Biotin Anti-GSTA1 antibody [8-326] (ab173835)
        All lanes : Biotin Anti-GSTA1 antibody [8-326] (ab173835) at 1/1000 dilution

        Lane 1 : Purified GSTA1 -SRC SH2 domain at 0.5 µg
        Lane 2 : Purified GSTA1 -SRC SH2 domain at 0.25 µg
        Lane 3 : Purified GSTA1-SRC SH2 domain at 0.125 µg
        Lane 4 : Purified GSTA1 -SRC SH2 domain at 0.06 µg
        Lane 5 : Purified GSTA1 -SRC SH2 domain at 0.03 µg

        Secondary
        All lanes : Streptavidin-HRP at 1/20000 dilution

        Developed using the ECL technique.

        Predicted band size: 26 kDa

      References (0)

      Publishing research using ab173835? Please let us know so that we can cite the reference in this datasheet.

      ab173835 has not yet been referenced specifically in any publications.

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