Biotin Anti-HA tag antibody [2-2.2.14] (ab173825)
![Western blot - Biotin Anti-HA tag antibody [2-2.2.14] (ab173825)](https://www.abcam.com/ps/products/173/ab173825/Images/ab173825-197545-anti-ha-tag-antibody-2-2214-biotin-western-blot.jpg "Western blot - Biotin Anti-HA tag antibody [2-2.2.14] (ab173825)")
Key features and details
- Biotin Mouse monoclonal [2-2.2.14] to HA tag
- Suitable for: IP, WB
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG1
Overview
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Product name
Biotin Anti-HA tag antibody [2-2.2.14]
See all HA tag primary antibodies -
Description
Biotin Mouse monoclonal [2-2.2.14] to HA tag -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to HA tag aa 1-9 conjugated to Ovalbumin.
Sequence:YPYDVPDYA
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Positive control
- E. coli lysate containing a multi-epitope tagged protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
2-2.2.14 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab173825 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
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| WB |
1/500 - 1/1000. Predicted molecular weight: 1 kDa.
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| Notes |
|---|
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IP
Use at an assay dependent concentration. |
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WB
1/500 - 1/1000. Predicted molecular weight: 1 kDa. |
Target
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Relevance
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
Alternative names
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
Images
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Western blot - Biotin Anti-HA tag antibody [2-2.2.14] (ab173825)All lanes : Biotin Anti-HA tag antibody [2-2.2.14] (ab173825) at 1/1000 dilution
Lane 1 : E. coli lysate containing a multi-epitope tagged protein at 0.25 µg
Lane 2 : E. coli lysate containing a multi-epitope tagged protein at 0.125 µg
Lane 3 : E. coli lysate containing a multi-epitope tagged protein at 0.0599999986588955 µg
Lane 4 : E. coli lysate containing a multi-epitope tagged protein at 0.0299999993294477 µg
Lane 5 : E. coli lysate containing a multi-epitope tagged protein at 0.0149999996647239 µg
Secondary
All lanes : Streptavidin-HRP at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 1 kDaNote: Predicted MW is for the HA tag alone.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab173825 has not yet been referenced specifically in any publications.
