Biotin Anti-HA tag antibody [2-2.2.14] (ab173825)

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Western blot - Biotin Anti-HA tag antibody [2-2.2.14] (ab173825)

    Key features and details

    • Biotin Mouse monoclonal [2-2.2.14] to HA tag
    • Suitable for: IP, WB
    • Reacts with: Species independent
    • Conjugation: Biotin
    • Isotype: IgG1

    Overview

    • Product name

      Biotin Anti-HA tag antibody [2-2.2.14]
      See all HA tag primary antibodies

    • Description

      Biotin Mouse monoclonal [2-2.2.14] to HA tag

    • Host species

      Mouse

    • Conjugation

      Biotin

    • Tested applications

      Suitable for: IP, WBmore details

    • Species reactivity

      Reacts with: Species independent

    • Immunogen

      Synthetic peptide corresponding to HA tag aa 1-9 conjugated to Ovalbumin.
      Sequence:

      YPYDVPDYA

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • E. coli lysate containing a multi-epitope tagged protein.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze.

    • Storage buffer

      Preservative: 0.02% Sodium azide
      Constituent: 99% PBS
    • Concentration information loading...

    • Purity

      Protein G purified

    • Clonality

      Monoclonal

    • Clone number

      2-2.2.14

    • Isotype

      IgG1

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab173825 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IP
    Use at an assay dependent concentration.
    WB
    1/500 - 1/1000. Predicted molecular weight: 1 kDa.
    Notes
    IP
    Use at an assay dependent concentration.
    WB
    1/500 - 1/1000. Predicted molecular weight: 1 kDa.

    Target

    • Relevance

      Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

    • Alternative names

      • HA epitope tag antibody
      • HA1 antibody
      • HA2 antibody
      • hemagglutinin antibody
      • Hemagglutinin HA1 chain antibody
      • Hemagglutinin HA2 chain antibody
      see all

    Images

    • Western blot - Biotin Anti-HA tag antibody [2-2.2.14] (ab173825)
      Western blot - Biotin Anti-HA tag antibody [2-2.2.14] (ab173825)
      All lanes : Biotin Anti-HA tag antibody [2-2.2.14] (ab173825) at 1/1000 dilution

      Lane 1 : E. coli lysate containing a multi-epitope tagged protein at 0.25 µg
      Lane 2 : E. coli lysate containing a multi-epitope tagged protein at 0.125 µg
      Lane 3 : E. coli lysate containing a multi-epitope tagged protein at 0.0599999986588955 µg
      Lane 4 : E. coli lysate containing a multi-epitope tagged protein at 0.0299999993294477 µg
      Lane 5 : E. coli lysate containing a multi-epitope tagged protein at 0.0149999996647239 µg

      Secondary
      All lanes : Streptavidin-HRP at 1/20000 dilution

      Developed using the ECL technique.

      Predicted band size: 1 kDa



      Note: Predicted MW is for the HA tag alone.

    References (0)

    Publishing research using ab173825? Please let us know so that we can cite the reference in this datasheet.

    ab173825 has not yet been referenced specifically in any publications.

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