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  1. Link

    biotin-ha-tag-antibody-ab26228.pdf

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Tags & Cell Markers Epitope Tags HA Tag
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Biotin Anti-HA tag antibody (ab26228)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (13)

Product price, shipping and contact information

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Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Biotin Rabbit polyclonal to HA tag
  • Suitable for: WB, ELISA
  • Reacts with: Species independent
  • Conjugation: Biotin
  • Isotype: IgG

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Overview

  • Product name

    Biotin Anti-HA tag antibody
    See all HA tag primary antibodies
  • Description

    Biotin Rabbit polyclonal to HA tag
  • Host species

    Rabbit
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    HA-tag conjugated to KLH

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: HEPES, 0.2% BSA
  • Concentration information loading...
  • Purification notes

    Immunoaffinity purified using the peptide conjugated to a solid-phase support
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • HA Tag

Associated products

  • Isotype control

    • Biotin Rabbit IgG - Isotype Control (ab200208)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab26228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/5000.
ELISA
1/5000 - 1/25000.
Notes
WB
1/1000 - 1/5000.
ELISA
1/5000 - 1/25000.

Target

  • Relevance

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names

    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (13)

Publishing research using ab26228? Please let us know so that we can cite the reference in this datasheet.

ab26228 has been referenced in 13 publications.

  • Thibado JK  et al. Differences in interactions between transmembrane domains tune the activation of metabotropic glutamate receptors. Elife 10:N/A (2021). PubMed: 33880992
  • Vyklicky V  et al. Conformational rearrangement of the NMDA receptor amino-terminal domain during activation and allosteric modulation. Nat Commun 12:2694 (2021). PubMed: 33976221
  • Poc P  et al. Interrogating surface versus intracellular transmembrane receptor populations using cell-impermeable SNAP-tag substrates. Chem Sci 11:7871-7883 (2020). PubMed: 34123074
  • Lee J  et al. Defining the Homo- and Heterodimerization Propensities of Metabotropic Glutamate Receptors. Cell Rep 31:107605 (2020). PubMed: 32375054
  • Kothari P  et al. Contractility kits promote assembly of the mechanoresponsive cytoskeletal network. J Cell Sci 132:N/A (2019). PubMed: 30559246
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Flow Cytometry abreview for Anti-HA tag antibody (Biotin)

Good
Abreviews
Abreviews
abreview image
Application
Flow Cytometry
Sample
Human Cell (A3.01 human CD4+ T cell line engineered to express)
Permeabilization
No
Gating Strategy
Gated on major population by FSC/SSC (i.e. excluding dead cells/debris)
Specification
A3.01 human CD4+ T cell line engineered to express
Preparation
Cell harvesting/tissue preparation method: Suspension cells
Sample buffer: RPMI 1640/10% FBS
Fixation
Formaldehyde
Read More

DR. Menelaos Symeonides

Verified customer

Submitted Mar 09 2020

Question

 Your product ab26228 is a biotinylated rabbit anti-HA polyclonal. Is that derived from the same rabbit anti-HA polyclonal as in ab9110?

Read More

Abcam community

Verified customer

Asked on Nov 17 2011

Answer

Yes, ab9110 is the unconjugated form of ab26228 (biotinylated form).

Read More

Abcam Scientific Support

Answered on Nov 17 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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