Biotin Anti-HBEGF/DTR antibody [4G10] (ab66793)

Submit a review Q&A (2)
Western blot - Biotin Anti-HBEGF/DTR antibody [4G10] (ab66793)
  • Immunocytochemistry/ Immunofluorescence - Biotin Anti-HBEGF/DTR antibody [4G10] (ab66793)
  • Immunoprecipitation - Biotin Anti-HBEGF/DTR antibody [4G10] (ab66793)

Key features and details

  • Biotin Mouse monoclonal [4G10] to HBEGF/DTR
  • Suitable for: IP, ICC/IF, WB
  • Reacts with: Human
  • Conjugation: Biotin
  • Isotype: IgG1

Overview

  • Product name

    Biotin Anti-HBEGF/DTR antibody [4G10]
    See all HBEGF/DTR primary antibodies

  • Description

    Biotin Mouse monoclonal [4G10] to HBEGF/DTR

  • Host species

    Mouse

  • Conjugation

    Biotin

  • Tested applications

    Suitable for: IP, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse

  • Immunogen

    Recombinant fragment corresponding to Human HBEGF/DTR.

  • Epitope

    EGF domain

  • Positive control

    • Vero cell extract carrying human HG-EGF expression vector.

  • General notes

    Biotin/IgG = 7.5

     This product was previously labelled as HB EGF

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab66793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
WB Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 20-28 kDa (predicted molecular weight: 23 kDa).

Target

  • Function

    May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts and smooth muscle but not endothelial cells. It is able to bind EGF receptors with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.

  • Sequence similarities

    Contains 1 EGF-like domain.

  • Post-translational
    modifications

    Several N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.
    O-linked glycan attachment sites were determined by Edman degradation, O-glycanase digest suggests mucin-type glycosylation (done in HB-EGF purified from histiocytic lymphoma cell line U-937).

  • Cellular localization

    Cell membrane and Secreted > extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor.
  • Information by UniProt

  • Database links

    • Alternative names

      • diphtheria toxin receptor (heparin-binding EGF-like growth factor) antibody
      • diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor) antibody
      • Diphtheria toxin receptor antibody
      • DT R antibody
      • DT-R antibody
      • DTR antibody
      • DTS antibody
      • DTSF antibody
      • HB-EGF antibody
      • HBEGF antibody
      • HBEGF_HUMAN antibody
      • HEGFL antibody
      • Heparin binding EGF like growth factor antibody
      • Heparin binding epidermal growth factor antibody
      • Heparin binding epidermal growth factor like growth factor antibody
      • Heparin-binding EGF-like growth factor antibody
      • Proheparin binding EGF like growth factor antibody
      see all

    Images

    • Western blot - Biotin Anti-HBEGF/DTR antibody [4G10] (ab66793)
      Western blot - Biotin Anti-HBEGF/DTR antibody [4G10] (ab66793)
      All lanes : Biotin Anti-HBEGF/DTR antibody [4G10] (ab66793) at 1 µg/ml

      Lane 1 : Vero cell extract
      Lane 2 : Vero cell extract from cells
      carrrying human HBEGF/DTR expression vector.
      Lane 3 : Vero cell extract from cells carrying
      mouse HBEGF/DTR expression vector.

      Predicted band size: 23 kDa
      Observed band size: 20-28 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 41 kDa. We are unsure as to the identity of these extra bands.

    • Immunocytochemistry/ Immunofluorescence - Biotin Anti-HBEGF/DTR antibody [4G10] (ab66793)
      Immunocytochemistry/ Immunofluorescence - Biotin Anti-HBEGF/DTR antibody [4G10] (ab66793)

      These images shows Vero cells carrying human HG-EGF expression vector (left hand image) and Vero cells carrying mouse HBEGF/DTR  expression vector (right hand image). Cells treated with ab66793, fixed with 4% PFA and reacted with Cys3 conjugated 2nd antibody.

    • Immunoprecipitation - Biotin Anti-HBEGF/DTR antibody [4G10] (ab66793)
      Immunoprecipitation - Biotin Anti-HBEGF/DTR antibody [4G10] (ab66793)

      All lanes : HBEGF/DTR  antibody [4G10](biotin) - Azide free (ab66793) at 1 µg/ml. Lane 1 : Vero cell extract Lane 2 : Vero cell extract from cells carrrying human HBEGF/DTR  expression vector. Lane 3 : Vero cell extract from cells carrying mouse HBEGF/DTR expression vector Predicted band size : 23 kDa Observed band size : 20-38 kDa Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands. This image shows Immunoprecipitation of HBEGF/DTR followed by Western blotting/SDS PAGE; the cell surface was biotinylated.

    References (0)

    Publishing research using ab66793? Please let us know so that we can cite the reference in this datasheet.

    ab66793 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    Is it possible to use both a biotin- and a mouse-specific secondary antibody on ab66793?  

    Read More
    Answer

    Thank you for your inquiry. You probably could use an anti-mouse secondary antibody with ab66793 since we know it worked well with a Cy3-conjugated secondary antibody. But, there are 7.5 biotin molecules per IgG1 molecule, and that could cause a lot of hindrance to block the binding of the secondary antibody. We do have anti-biotin conjugated antibodies (if that's what you are looking for), such as Anti-Biotin (Alkaline Phosphatase) ab6652, Anti-Biotin (HRP) ab19221, and Anti-Biotin (FITC) ab6650. Click here (or use the following: https://www.abcam.com/Alkaline-Phosphatase-Biotin-antibody-ab6652.html). Click here (or use the following: https://www.abcam.com/HRP-Biotin-antibody-ab19221.html). Click here (or use the following: https://www.abcam.com/FITC-Biotin-antibody-ab6650.html). What is probably best to do is to use a strepatvidin conjugate to bind the biotin on ab66793. We have two (peroxidase and AP). https://www.abcam.com/Streptavidin-Peroxidase-Ready-to-Use-ab64269.html https://www.abcam.com/Streptavidin-Alkaline-Phosphatase-Ready-to-Use-ab64268.html I hope this information helps. Please contact us with any other questions.

    Read More

    Question

    I have a question regarding ab66793. Does this antibody have cross-reactivity with Monkey, like Macaque?

    Read More
    Answer

    We could not find the sequence of Macaque monkey HB-EGF in databases. However, since the green monkey HB-EGF sequence has 97% identity to that of human, there is a good chance that ab66793 react with Macaque HB-EGF. Though the human HB-EGF has 80% identity with that of mouse and ab66793 did not react with mouse HB-EGF.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com