Biotin Anti-Hepatitis C Virus antibody [NS5B-6] (ab122972)
![Western blot - Biotin Anti-Hepatitis C Virus antibody [NS5B-6] (ab122972)](https://www.abcam.com/ps/products/122/ab122972/Images/Hepatitis-C-Virus-Primary-antibodies-ab122972-2.jpg "Western blot - Biotin Anti-Hepatitis C Virus antibody [NS5B-6] (ab122972)")
Key features and details
- Biotin Mouse monoclonal [NS5B-6] to Hepatitis C Virus
- Suitable for: WB, ELISA, Flow Cyt, ICC/IF
- Reacts with: Hepatitis C virus
- Conjugation: Biotin
- Isotype: IgG2b
Overview
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Product name
Biotin Anti-Hepatitis C Virus antibody [NS5B-6]
See all Hepatitis C Virus primary antibodies -
Description
Biotin Mouse monoclonal [NS5B-6] to Hepatitis C Virus -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: WB, ELISA, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Hepatitis C virus -
Immunogen
Recombinant fragment of NS5b protein of Hepatitis C Virus genotype 1b, expressed in E. coli
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Positive control
- Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA.
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General notes
Conjugate: Biotin conjugated [biotin] / [IgG] = 8.7
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Purification notes
filter-sterilized -
Clonality
Monoclonal -
Clone number
NS5B-6 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab122972 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 70 kDa). | |
| ELISA | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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| ICC/IF | Use at an assay dependent concentration. |
Target
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Relevance
Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha. -
Cellular localization
Virus surface -
Database links
- Entrez Gene: 951475 Hepatitis C virus
- SwissProt: P27958 Hepatitis C virus
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Alternative names
- Genome polyprotein antibody
- HCV antibody
- Hepatitis C virus polyprotein antibody
Images
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Western blot - Biotin Anti-Hepatitis C Virus antibody [NS5B-6] (ab122972)Biotin Anti-Hepatitis C Virus antibody [NS5B-6] (ab122972) + Lysate of chimp liver cells infected with recombinant
vaccinia virus containing a HCV genome cDNA
Predicted band size: 70 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
NS5b protein is detected as a 58kDa band. -
![Immunocytochemistry/ Immunofluorescence - Biotin Anti-Hepatitis C Virus antibody [NS5B-6] (ab122972)](https://www.abcam.com/ps/products/122/ab122972/Images/Hepatitis-C-Virus-Primary-antibodies-ab122972-3.jpg)
Immunocytochemistry/ Immunofluorescence - Biotin Anti-Hepatitis C Virus antibody [NS5B-6] (ab122972)ab122972, staining Hepatitis C Virus NS5b protein in Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA by immunofluorescence.
Protocols
Datasheets and documents
References (2)
ab122972 has been referenced in 2 publications.
- Zhang MQ et al. Differential Effects of Autophagy-Related 10 Protein on HCV Replication and Autophagy Flux Are Mediated by Its Cysteine44 and Cysteine135. Front Immunol 9:2176 (2018). PubMed: 30319633
- Manabe S et al. Production of nonstructural proteins of hepatitis C virus requires a putative viral protease encoded by NS3. Virology 198:636-44 (1994). PubMed: 8291245
