Key features and details
- Biotin Mouse monoclonal [H6-29] to Hepatitis C Virus Core Antigen
- Suitable for: ICC/IF, WB, IHC-Fr
- Reacts with: Hepatitis C virus
- Conjugation: Biotin
- Isotype: IgG2a
Product nameBiotin Anti-Hepatitis C Virus Core Antigen antibody [H6-29]
See all Hepatitis C Virus Core Antigen primary antibodies
DescriptionBiotin Mouse monoclonal [H6-29] to Hepatitis C Virus Core Antigen
Tested applicationsSuitable for: ICC/IF, WB, IHC-Frmore details
Species reactivityReacts with: Hepatitis C virus
Recombinant fragment within Hepatitis C Virus Hepatitis C Virus Core Antigen aa 13-124 (internal sequence). The exact sequence is proprietary. corresponding to part of the Core protein region. Expressed in E. coli.
RNTNRRPQDVKFPGGGQIVGGVYLLPRRGPRLGVRAPRKTSERSQPRGRR QPIPKARRPE GRTWAQPGYPWPLYGNEGLGWAGWLLSPRGSRPSWGPT DPRRRSRNLGKVID
Database link: P26663
- Chimpanzee liver infected with recombinant vaccinia virus containing a Hepatitis C virus genome cDNA lysate and cells. Human liver tissue from a patient with chronic hepatitis C.
Biotin conjugated [biotin] / [IgG] = 6.6.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 6
Constituents: 50% PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein A purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab192050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).|
|IHC-Fr||Use at an assay dependent concentration.|
RelevanceThe hepatitis C virus (HCV) core protein represents the first 191 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum. Hepatitis C virus (HCV) core is a viral structural protein; it also participates in some cellular processes, including transcriptional regulation. However the mechanisms of core-mediated transcriptional regulation remain poorly understood. Hepatitis C virus (HCV) core protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways. In addition, HCV core antigen is a recently developed marker of hepatitis C infection. The HCV core protein has been previously shown to circulate in the bloodstream of HCV-infected patients and inhibit host immunity through an interaction with gC1qR. Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Hepatitis C virus (HCV) causes most cases of non-A, non-B hepatitis and results in most HCV infected people developing chronic infections, liver cirrhosis and hepatocellular carcinoma. T cell responses, including interferon-gamma production are severely suppressed in chronic HCV patients.
Cellular localizationEndoplasmic reticulum
- Core protein p21 antibody
- HCV core antigen antibody
- HCV core protein antibody
- Hepatitis C Virus core protein antibody
Biotin Anti-Hepatitis C Virus Core Antigen antibody [H6-29] (ab192050) + chimpanzee liver infected with recombinant vaccinia virus containing a Hepatitis C virus genome cDNA cell lysate
Predicted band size: 22 kDa
Observed band size: 22 kDa
Immunofluorescence analysis of acetone-fixed cells from chimpanzee liver infected with recombinant vaccinia virus containing a Hepatitis C virus genome cDNA cells, labeling Hepatitis C Virus NS4A using ab192050.
Immunohistochemical analysis of Human liver tissue from a patient with chronic hepatitis C, labeling Hepatitis C Virus NS4A using ab192050. Counterstained with hematoxylin.
ab192050 has not yet been referenced specifically in any publications.