Key features and details
- Biotin Mouse monoclonal [S4-13] to Hepatitis C Virus NS4A
- Suitable for: WB, ELISA, Flow Cyt, ICC/IF
- Reacts with: Hepatitis C virus
- Conjugation: Biotin
- Isotype: IgG2b
Product nameBiotin Anti-Hepatitis C Virus NS4A antibody [S4-13]
See all Hepatitis C Virus NS4A primary antibodies
DescriptionBiotin Mouse monoclonal [S4-13] to Hepatitis C Virus NS4A
SpecificitySpecific to Human Hepatitis C Virus NS4A protein of genotype 1b. Not tested in other genotypes
Tested applicationsSuitable for: WB, ELISA, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Hepatitis C virus
A region of NS4 protein of Hepatitis C Virus genotype 1b expressed in E. coli.
EpitopeN terminal region of the NS4 protein (NS4A).
- Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA.
[biotin] / [IgG] = 11.3
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 6
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
Purification notesPurity >95% by SDS-PAGE.
Light chain typekappa
Our Abpromise guarantee covers the use of ab124526 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 6 kDa.|
|ELISA||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration.|
RelevanceHepatitis C Virus is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non-structural protein necessary for viral replication. Several different genotypes of Hepatitis C Virus with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha
Cellular localizationHost endoplasmic reticulum membrane; Single-pass type I membrane protein Potential. Note: Host membrane insertion occurs after processing by the NS3 protease.
- core protein antibody
- E1 protein antibody
- E2 protein antibody
Biotin Anti-Hepatitis C Virus NS4A antibody [S4-13] (ab124526) + Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA.
Predicted band size: 6 kDa
ab124526 staining Hepatitis C Virus NS4A in Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA. After incubation for 48 hr, the cells were fixed with acetone and Hepatitis C Virus NS4A protein was detected by indirect immunofluorescence staining using this antibody.
ab124526 has not yet been referenced specifically in any publications.