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    biotin-histone-h4-acetyl-k5-antibody-ep1000y-ab202886.pdf

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Epigenetics and Nuclear Signaling Histones H4 Acetylated
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RecombinantRabMAb

Recombinant Biotin Anti-Histone H4 (acetyl K5) antibody [EP1000Y] (ab202886)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Histone H4 (acetyl K5) antibody [EP1000Y] (ab202886)
  • Biotin Anti-Histone H4 (acetyl K5) antibody [EP1000Y] (ab202886)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Biotin Rabbit monoclonal [EP1000Y] to Histone H4 (acetyl K5)
  • Suitable for: IHC-P
  • Reacts with: Human
  • Conjugation: Biotin

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Protein
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Recombinant Human Histone H4 protein (ab198115)
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Overview

  • Product name

    Biotin Anti-Histone H4 (acetyl K5) antibody [EP1000Y]
    See all Histone H4 primary antibodies
  • Description

    Biotin Rabbit monoclonal [EP1000Y] to Histone H4 (acetyl K5)
  • Host species

    Rabbit
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Xenopus laevis, Rice
  • Immunogen

    Synthetic peptide within Human Histone H4 (N terminal) (acetyl K5). The exact sequence is proprietary.

  • Positive control

    • IHC-P: Human normal colon FFPE tissue sections.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1000Y
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • H4
    • Acetylated

Associated products

  • Alternative Versions

    • Anti-Histone H4 (acetyl K5) antibody [EP1000Y] - BSA and Azide free (ab232507)
    • Anti-Histone H4 (acetyl K5) antibody [EP1000Y] - ChIP Grade (ab51997)
  • Isotype control

    • Biotin Rabbit IgG - Isotype Control (ab200208)
  • Recombinant Protein

    • Recombinant Human Histone H4 protein (ab198115)

Applications

Our Abpromise guarantee covers the use of ab202886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similarities

    Belongs to the histone H4 family.
  • Post-translational
    modifications

    Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
    Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
    Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
    Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
    Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
    Sumoylated, which is associated with transcriptional repression.
  • Cellular localization

    Nucleus. Chromosome.
  • Target information above from: UniProt accession P62805 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 121504 Human
    • Entrez Gene: 554313 Human
    • Entrez Gene: 8294 Human
    • Entrez Gene: 8359 Human
    • Entrez Gene: 8360 Human
    • Entrez Gene: 8361 Human
    • Entrez Gene: 8362 Human
    • Entrez Gene: 8363 Human
    • Entrez Gene: 8364 Human
    • Entrez Gene: 8365 Human
    • Entrez Gene: 8366 Human
    • Entrez Gene: 8367 Human
    • Entrez Gene: 8368 Human
    • Entrez Gene: 8370 Human
    • Entrez Gene: 100041230 Mouse
    • Entrez Gene: 319155 Mouse
    • Entrez Gene: 319156 Mouse
    • Entrez Gene: 319157 Mouse
    • Entrez Gene: 319158 Mouse
    • Entrez Gene: 319159 Mouse
    • Entrez Gene: 319160 Mouse
    • Entrez Gene: 319161 Mouse
    • Entrez Gene: 320332 Mouse
    • Entrez Gene: 326619 Mouse
    • Entrez Gene: 326620 Mouse
    • Entrez Gene: 674678 Mouse
    • Entrez Gene: 69386 Mouse
    • Entrez Gene: 97122 Mouse
    • Entrez Gene: 64627 Rat
    • Entrez Gene: 447787 Xenopus laevis
    • Omim: 142750 Human
    • Omim: 602822 Human
    • Omim: 602823 Human
    • Omim: 602824 Human
    • Omim: 602825 Human
    • Omim: 602826 Human
    • Omim: 602827 Human
    • Omim: 602828 Human
    • Omim: 602829 Human
    • Omim: 602830 Human
    • Omim: 602831 Human
    • Omim: 602833 Human
    • SwissProt: P62805 Human
    • SwissProt: P62806 Mouse
    • SwissProt: P62804 Rat
    • SwissProt: P62799 Xenopus laevis
    • Unigene: 143080 Human
    • Unigene: 247816 Human
    • Unigene: 248172 Human
    • Unigene: 248178 Human
    • Unigene: 248179 Human
    • Unigene: 278483 Human
    • Unigene: 46423 Human
    • Unigene: 528055 Human
    • Unigene: 533295 Human
    • Unigene: 55468 Human
    • Unigene: 591790 Human
    • Unigene: 655235 Human
    • Unigene: 662174 Human
    • Unigene: 706635 Human
    • Unigene: 718935 Human
    • Unigene: 144300 Mouse
    • Unigene: 14775 Mouse
    • Unigene: 158272 Mouse
    • Unigene: 227295 Mouse
    • Unigene: 228709 Mouse
    • Unigene: 246720 Mouse
    • Unigene: 255646 Mouse
    • Unigene: 260530 Mouse
    • Unigene: 261642 Mouse
    • Unigene: 261662 Mouse
    • Unigene: 261664 Mouse
    • Unigene: 377875 Mouse
    • Unigene: 442307 Mouse
    • Unigene: 85592 Xenopus laevis
    see all
  • Alternative names

    • dJ160A22.1 antibody
    • dJ160A22.2 antibody
    • dJ221C16.1 antibody
    • dJ221C16.9 antibody
    • FO108 antibody
    • H4 antibody
    • H4.k antibody
    • H4/a antibody
    • H4/b antibody
    • H4/c antibody
    • H4/d antibody
    • H4/e antibody
    • H4/g antibody
    • H4/h antibody
    • H4/I antibody
    • H4/j antibody
    • H4/k antibody
    • H4/m antibody
    • H4/n antibody
    • H4/p antibody
    • H4_HUMAN antibody
    • H4F2 antibody
    • H4F2iii antibody
    • H4F2iv antibody
    • H4FA antibody
    • H4FB antibody
    • H4FC antibody
    • H4FD antibody
    • H4FE antibody
    • H4FG antibody
    • H4FH antibody
    • H4FI antibody
    • H4FJ antibody
    • H4FK antibody
    • H4FM antibody
    • H4FN antibody
    • H4M antibody
    • HIST1H4A antibody
    • HIST1H4B antibody
    • HIST1H4C antibody
    • HIST1H4D antibody
    • HIST1H4E antibody
    • HIST1H4F antibody
    • HIST1H4H antibody
    • HIST1H4I antibody
    • HIST1H4J antibody
    • HIST1H4K antibody
    • HIST1H4L antibody
    • HIST2H4 antibody
    • HIST2H4A antibody
    • Hist4h4 antibody
    • Histone 1 H4a antibody
    • Histone 1 H4b antibody
    • Histone 1 H4c antibody
    • Histone 1 H4d antibody
    • Histone 1 H4e antibody
    • Histone 1 H4f antibody
    • Histone 1 H4h antibody
    • Histone 1 H4i antibody
    • Histone 1 H4j antibody
    • Histone 1 H4k antibody
    • Histone 1 H4l antibody
    • Histone 2 H4a antibody
    • histone 4 H4 antibody
    • Histone H4 antibody
    • MGC24116 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Histone H4 (acetyl K5) antibody [EP1000Y] (ab202886)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Histone H4 (acetyl K5) antibody [EP1000Y] (ab202886)

    IHC image of Histone H4 (acetyl K5) staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins, before blocking of endogenous biotin. The section was then incubated with ab202886, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Biotin Anti-Histone H4 (acetyl K5) antibody [EP1000Y] (ab202886)
    Biotin Anti-Histone H4 (acetyl K5) antibody [EP1000Y] (ab202886)

Protocols

  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab202886? Please let us know so that we can cite the reference in this datasheet.

    ab202886 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab202886.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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