RecombinantRabMAb

Recombinant Biotin Anti-Histone H4 (acetyl K5) antibody [EP1000Y] (ab202886)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Histone H4 (acetyl K5) antibody [EP1000Y] (ab202886)
  • Biotin Anti-Histone H4 (acetyl K5) antibody [EP1000Y] (ab202886)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Biotin Rabbit monoclonal [EP1000Y] to Histone H4 (acetyl K5)
  • Suitable for: IHC-P
  • Reacts with: Human
  • Conjugation: Biotin

Overview

  • Product name

    Biotin Anti-Histone H4 (acetyl K5) antibody [EP1000Y]
    See all Histone H4 primary antibodies

  • Description

    Biotin Rabbit monoclonal [EP1000Y] to Histone H4 (acetyl K5)

  • Host species

    Rabbit

  • Conjugation

    Biotin

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Xenopus laevis, Rice

  • Immunogen

    Synthetic peptide within Human Histone H4 (N terminal) (acetyl K5). The exact sequence is proprietary.

  • Positive control

    • IHC-P: Human normal colon FFPE tissue sections.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP1000Y

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab202886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Histone H4 (acetyl K5) antibody [EP1000Y] (ab202886)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Histone H4 (acetyl K5) antibody [EP1000Y] (ab202886)

    IHC image of Histone H4 (acetyl K5) staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins, before blocking of endogenous biotin. The section was then incubated with ab202886, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Biotin Anti-Histone H4 (acetyl K5) antibody [EP1000Y] (ab202886)
    Biotin Anti-Histone H4 (acetyl K5) antibody [EP1000Y] (ab202886)

References (0)

Publishing research using ab202886? Please let us know so that we can cite the reference in this datasheet.

ab202886 has not yet been referenced specifically in any publications.

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