Key features and details
- Biotin Rabbit polyclonal to HIV1 tat
- Suitable for: WB, ELISA, Flow Cyt
- Conjugation: Biotin
- Isotype: IgG
Product nameBiotin Anti-HIV1 tat antibody
See all HIV1 tat primary antibodies
DescriptionBiotin Rabbit polyclonal to HIV1 tat
Tested applicationsSuitable for: WB, ELISA, Flow Cytmore details
Species reactivityReacts with: Other species
Recombinant full length protein expressed in E. coli
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 0.05% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab43015 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: 1/200. Use Streptavidin-FITC conjugated detection reagent.
WB: 1/1000. Predicted molecular weight: 14 kDa. Use Streptavidin (HRP) conjugated detection reagent.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceHIV1 tat (Transactivator of Transcription) protein is a pleiotropic factor that induces a broad range of biological effects in numerous cell types. At the HIV promoter, tat is a powerful transactivator of gene expression which acts by inducing chromatin remodeling and by recruiting elongation-competent transcriptional complexes on to the viral LTR.
Cellular localizationNuclear. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region.
- p14 antibody
- Protein Tat antibody
- Transactivating regulatory protein antibody
ab43015 has been referenced in 2 publications.
- Dickens AM et al. Chronic low-level expression of HIV-1 Tat promotes a neurodegenerative phenotype with aging. Sci Rep 7:7748 (2017). ELISA . PubMed: 28798382
- Johnson TP et al. Induction of IL-17 and nonclassical T-cell activation by HIV-Tat protein. Proc Natl Acad Sci U S A 110:13588-93 (2013). PubMed: 23898208