Biotin Anti-HLA Class I antibody [W6/32] (ab110665)

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Key features and details

  • Biotin Mouse monoclonal [W6/32] to HLA Class I
  • Reacts with: Cow, Cat, Human, Non human primates
  • Conjugation: Biotin
  • Isotype: IgG2a

Overview

  • Product name

    Biotin Anti-HLA Class I antibody [W6/32]
    See all HLA Class I primary antibodies

  • Description

    Biotin Mouse monoclonal [W6/32] to HLA Class I

  • Host species

    Mouse

  • Conjugation

    Biotin

  • Species reactivity

    Reacts with: Cow, Cat, Human, Non human primates
    Does not react with: Rabbit

  • Immunogen

    Tissue, cells or virus corresponding to Human HLA Class I.

  • General notes

    Suitable as a positive control for HLA tissue typing and crossmatching.

    The purified antibody (>95% by SDS-PAGE) is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Size exclusion

  • Primary antibody notes

    Suitable as a positive control for HLA tissue typing and crossmatching.

  • Clonality

    Monoclonal

  • Clone number

    W6/32

  • Isotype

    IgG2a

  • Research areas

Target

References (4)

Publishing research using ab110665? Please let us know so that we can cite the reference in this datasheet.

ab110665 has been referenced in 4 publications.

  • Wang D  et al. Targeted Disruption of the ß2-Microglobulin Gene Minimizes the Immunogenicity of Human Embryonic Stem Cells. Stem Cells Transl Med 4:1234-45 (2015). PubMed: 26285657
  • Choudhuri K  et al. Peptide-major histocompatibility complex dimensions control proximal kinase-phosphatase balance during T cell activation. J Biol Chem 284:26096-105 (2009). PubMed: 19628870
  • Tran TM  et al. The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex. Immunogenetics 53:440-6 (2001). PubMed: 11685454
  • Kievits F & Ivanyi P Monomorphic anti-HLA monoclonal antibody (W6/32) recognizes polymorphic H-2 heavy-chain determinants exposed by association with bovine or human but not murine beta 2-microglobulin. Hum Immunol 20:115-26 (1987). PubMed: 3316135

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

I will go ahead and order the product today. I just have a questions. What if I dont want to order the second product and still want to use the discount code. Do I have to buy a second product

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Answer

Thank you for your reply. To take advantage of the testing discount promotion, you must first purchase the antibody (in this case ab21148) at full price + shipping and handling. You then test the antibody in your sELISA and submit an Abreview on the results that you obtained. These results could be positive or negative, we just want as much information as we can. After you have submitted the Abreview, the testing discount code that I sent will become active and you can then go ahead and use the code to get an antibody free of charge from our catalogue. I hope this helps clarify how the discount program works. You can find all of these details in the link below: https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount Please let me know if there is anything else I can help you with, or if you have any further questions about the testing discount program.

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Question

I am looking for a monoclonal antibody W6/32 -biotin(Biotinylated W6/32) which can work in sandwich ELISA. Please let me know if you have any such antibody. I looked for these antibodies on your websites and all of them have just flow cytometry applications

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Answer

Thank you for contacting Abcam. The only monoclonal antibodies we have to HLA Class I antibody [W6/32] (Biotin) are ab21148 and ab110665. As you mentioned neither have been shown to work in ELISA, therefore both are eligible for our testing discount program. Details of this can be found below: https://www.abcam.com/index.htmlpageconfig=resource&rid=11998&viapagetrap=collaborationdiscount If you would like to take advantage of this testing discount program, or if there is anything else I can help you with, please let me know.

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