Biotin Anti-HLA DR + HLA DP antibody [MEM-136] (ab239239)

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Flow Cytometry - Biotin Anti-HLA DR + HLA DP antibody [MEM-136] (ab239239)

    Key features and details

    • Biotin Mouse monoclonal [MEM-136] to HLA DR + HLA DP
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: Biotin
    • Isotype: IgG1

    Overview

    • Product name

      Biotin Anti-HLA DR + HLA DP antibody [MEM-136]
      See all HLA DR + HLA DP primary antibodies

    • Description

      Biotin Mouse monoclonal [MEM-136] to HLA DR + HLA DP

    • Host species

      Mouse

    • Conjugation

      Biotin

    • Specificity

      ab239239 recognizes a common epitope on beta-chain of human HLA-DR and HLA-DP. It reacts with alpha/beta dimer as well as with dissociated beta-subunit.

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Tissue, cells or virus corresponding to Human HLA DR + HLA DP. (PHA-activated peripheral blood lymphocytes).

    • Positive control

      • Flow Cyt: Human peripheral blood.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.

    • Storage buffer

      pH: 7.40
      Preservative: 0.0975% Sodium azide
      Constituent: PBS
    • Concentration information loading...

    • Purity

      Size exclusion

    • Purification notes

      Purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

    • Clonality

      Monoclonal

    • Clone number

      MEM-136

    • Isotype

      IgG1

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab239239 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt 1/400.

    Target

    • Relevance

      Human MHC class II antigens are transmembrane glycoproteins composed of an alpha (36 kDa) and a beta chain (27kDa) and are expressed primarily on antigen presenting cells. Human MHC class II genes are located in the HLA-D region that encodes at least 6 alpha and 10 beta chain genes. Three loci, DR, DQ and DP, encode the major products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells and therefore have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.

    • Cellular localization

      Cell Membrane; Single pass type I membrane protein

    • Database links

      see all

    • Alternative names

      • HLA DP antibody
      • HLA DR antibody
      • MHC Class II antibody

    Images

    • Flow Cytometry - Biotin Anti-HLA DR + HLA DP antibody [MEM-136] (ab239239)
      Flow Cytometry - Biotin Anti-HLA DR + HLA DP antibody [MEM-136] (ab239239)

      Flow cytometric analysis of human peripheral blood labeling HLA DR + HLA DP with ab239239 at 1/400 dilution. Surface staining. Gated on leukocytes.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab239239? Please let us know so that we can cite the reference in this datasheet.

    ab239239 has not yet been referenced specifically in any publications.

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