Biotin Anti-HLA G antibody [MEM-G/1] (ab26034)
![Western blot - Biotin Anti-HLA G antibody [MEM-G/1] (ab26034)](https://www.abcam.com/ps/products/26/ab26034/Images/ab26034-278875-anti-hla-g-antibody-mem-g-1-biotin-western-blot.jpg "Western blot - Biotin Anti-HLA G antibody [MEM-G/1] (ab26034)")
Key features and details
- Biotin Mouse monoclonal [MEM-G/1] to HLA G
- Suitable for: WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Overview
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Product name
Biotin Anti-HLA G antibody [MEM-G/1]
See all HLA G primary antibodies -
Description
Biotin Mouse monoclonal [MEM-G/1] to HLA G -
Host species
Mouse -
Conjugation
Biotin -
Specificity
ab26034 reacts with denaturated HLA-G heavy chain. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant HLA-G denaturated heavy chain (Human)
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General notes
The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MEM-G/1 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab26034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/100. Predicted molecular weight: 38 kDa. |
Target
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Function
Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells. -
Tissue specificity
Expressed in trophoblasts. -
Sequence similarities
Belongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3135 Human
- Omim: 142871 Human
- SwissProt: P17693 Human
- Unigene: 512152 Human
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Alternative names
- B2 microglobulin antibody
- DADB-15K14.8 antibody
- HLA 6.0 antibody
see all
Images
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Western blot - Biotin Anti-HLA G antibody [MEM-G/1] (ab26034)Western Blot of HLA-G1 transfectant cells labeling HLA G with Anti-HLA G antibody [MEM-G/1] (Biotin) (ab26034).
References (4)
ab26034 has been referenced in 4 publications.
- Le Page ME et al. Killer Ig-like receptor 2DL4 does not mediate NK cell IFN-? responses to soluble HLA-G preparations. J Immunol 192:732-40 (2014). PubMed: 24337374
- Boyson JE et al. Disulfide bond-mediated dimerization of HLA-G on the cell surface. Proc Natl Acad Sci U S A 99:16180-5 (2002). PubMed: 12454284
- Fournel S et al. Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules. Tissue Antigens 55:510-8 (2000). PubMed: 10902607
- Frumento G et al. Melanomas and melanoma cell lines do not express HLA-G, and the expression cannot be induced by gammaIFN treatment. Tissue Antigens 56:30-7 (2000). PubMed: 10958353
