Biotin Anti-HLA G antibody [MEM-G/1] (ab26034)
![Western blot - Biotin Anti-HLA G antibody [MEM-G/1] (ab26034)](https://www.abcam.com/ps/products/26/ab26034/Images/ab26034-278875-anti-hla-g-antibody-mem-g-1-biotin-western-blot.jpg "Western blot - Biotin Anti-HLA G antibody [MEM-G/1] (ab26034)")
Key features and details
- Biotin Mouse monoclonal [MEM-G/1] to HLA G
- Suitable for: WB
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Biotin Anti-HLA G antibody [MEM-G/1]
See all HLA G primary antibodies -
Description
Biotin Mouse monoclonal [MEM-G/1] to HLA G -
Host species
Mouse -
Conjugation
Biotin -
Specificity
ab26034 reacts with denaturated HLA-G heavy chain. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Recombinant HLA-G denaturated heavy chain (Human)
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General notes
The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MEM-G/1 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab26034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/100. Predicted molecular weight: 38 kDa.
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| Notes |
|---|
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WB
1/100. Predicted molecular weight: 38 kDa. |
Target
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Function
Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells. -
Tissue specificity
Expressed in trophoblasts. -
Sequence similarities
Belongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- B2 microglobulin antibody
- DADB-15K14.8 antibody
- HLA 6.0 antibody
see all
Images
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Western blot - Biotin Anti-HLA G antibody [MEM-G/1] (ab26034)Western Blot of HLA-G1 transfectant cells labeling HLA G with Anti-HLA G antibody [MEM-G/1] (Biotin) (ab26034).
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab26034 has been referenced in 4 publications.
- Le Page ME et al. Killer Ig-like receptor 2DL4 does not mediate NK cell IFN-? responses to soluble HLA-G preparations. J Immunol 192:732-40 (2014). PubMed: 24337374
- Boyson JE et al. Disulfide bond-mediated dimerization of HLA-G on the cell surface. Proc Natl Acad Sci U S A 99:16180-5 (2002). PubMed: 12454284
- Fournel S et al. Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules. Tissue Antigens 55:510-8 (2000). PubMed: 10902607
- Frumento G et al. Melanomas and melanoma cell lines do not express HLA-G, and the expression cannot be induced by gammaIFN treatment. Tissue Antigens 56:30-7 (2000). PubMed: 10958353