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Immunology Immunoglobulins Heavy Chain IgG
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Recombinant

Recombinant Biotin Anti-Human Ig light chain antibody [RM129] (ab193185)

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Sandwich ELISA - Biotin Anti-Human Ig light chain antibody [RM129] (ab193185)
  • ELISA - Biotin Anti-Human Ig light chain antibody [RM129] (ab193185)
  • Biotin Anti-Human Ig light chain antibody [RM129] (ab193185)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Biotin Rabbit monoclonal [RM129] to Human Ig light chain
  • Suitable for: Sandwich ELISA, ELISA
  • Reacts with: Human
  • Conjugation: Biotin

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Overview

  • Product name

    Biotin Anti-Human Ig light chain antibody [RM129]
    See all Human Ig light chain primary antibodies
  • Description

    Biotin Rabbit monoclonal [RM129] to Human Ig light chain
  • Host species

    Rabbit
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: Sandwich ELISA, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat, Goat
  • Immunogen

    Full length protein corresponding to Human IgG. Purified Native Human IgG from Human Plasma

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 48.91% PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Protein A affinity purified from an animal origin–free culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    RM129
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Immunology
    • Immunoglobulins
    • Light Chain
    • Kappa
    • Immunology
    • Immunoglobulins
    • Light Chain
    • Lambda

Associated products

  • Isotype control

    • Biotin Rabbit IgG - Isotype Control (ab200208)

Applications

Our Abpromise guarantee covers the use of ab193185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 0.02 - 0.25 µg/ml.

May be paired with ab195574.

ELISA Use at an assay dependent concentration.

Target

  • Relevance

    There are only two types of light chain: kappa and lambda in mammals. Other types of light chains are found in lower vertebrates as the Ig-Light-Iota chain in Chondrichthyes and Teleostei. In each antibody, only one type is present and the two chains are identical. Each light chain has two successive domains: one constant and one variable domain. In humans 60% of light chains are kappa and 40% lambda,whereas in the mouse 95% of light chains are kappa. The amino acid sequences of lambda chains vary slightly at a few positions, allowing them to be classified into subtypes. The number of subtypes varies between species. Monoclonal immunoglobulin free light chains (FLC) are found in the serum and urine (Bence-Jones protein) of patients with a number of B-cell proliferative disorders, including multiple myeloma. Changes in serum FLC concentrations can provide a rapid and sensitive indication of response to treatment.
  • Cellular localization

    Cell Membrane: single-pass type I membrane protein and Secreted
  • Database links

    • Entrez Gene: 3514 Human
    • Omim: 147200 Human
    • SwissProt: P01834 Human
    • SwissProt: Q502W4 Human
    • SwissProt: Q569I9 Human
    • SwissProt: Q6GMV9 Human
    • SwissProt: Q6GMW1 Human
    • SwissProt: Q6GMX8 Human
    • SwissProt: Q6GMX9 Human
    • SwissProt: Q6P5R5 Human
    • SwissProt: Q6PIH7 Human
    • SwissProt: Q6PIT5 Human
    • SwissProt: Q7Z473 Human
    see all
  • Alternative names

    • HCAK1 antibody
    • Ig kappa chain antibody
    • IGKC antibody
    • Immunoglobulin kappa constant antibody
    • immunoglobulin kappa light chain (VJ) antibody
    • kappa 1 immunoglobulin light chain antibody
    • KM antibody
    • MGC111575 antibody
    • MGC62011 antibody
    • MGC72072 antibody
    • MGC88770 antibody
    • MGC88771 antibody
    • MGC88809 antibody
    see all

Images

  • Sandwich ELISA - Biotin Anti-Human Ig light chain antibody [RM129] (ab193185)
    Sandwich ELISA - Biotin Anti-Human Ig light chain antibody [RM129] (ab193185)

    A titer Sandwich ELISA using ab193185 as the detection antibody. The plate, coated with the capture antibody anti-human IgG, was loaded with different amounts of human IgG. A serial dilution of ab193185 was used as the detection antibody, followed with an alkaline phosphatase conjugated streptavidin.

  • ELISA - Biotin Anti-Human Ig light chain antibody [RM129] (ab193185)
    ELISA - Biotin Anti-Human Ig light chain antibody [RM129] (ab193185)

    ELISA of human immunoglobulins shows ab193185 reacts to both kappa and lambda light chains of human immunoglobulins. No cross reactivity with mouse, rat, or goat immunoglobulin light chain. The plate was coated with 50ng/well of different immunglobulins. 100 ng/mL or 20 ng/mL was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • Biotin Anti-Human Ig light chain antibody [RM129] (ab193185)
    Biotin Anti-Human Ig light chain antibody [RM129] (ab193185)

Protocols

  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab193185? Please let us know so that we can cite the reference in this datasheet.

    ab193185 has not yet been referenced specifically in any publications.

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