Recombinant Biotin Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab201490)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Biotin Rabbit monoclonal [EPR5539-71-8] to Human Kappa Chain
- Suitable for: IHC-P, ELISA
- Reacts with: Human
- Conjugation: Biotin
Overview
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Product name
Biotin Anti-Human Kappa Chain antibody [EPR5539-71-8]
See all Human Kappa Chain primary antibodies -
Description
Biotin Rabbit monoclonal [EPR5539-71-8] to Human Kappa Chain -
Host species
Rabbit -
Conjugation
Biotin -
Tested applications
Suitable for: IHC-P, ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Other Immunogen Type corresponding to Human Kappa Chain.
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Positive control
- IHC-P: Human normal tonsil tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EPR5539-71-8 -
Isotype
IgG -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
- Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)
- Alexa Fluor® 647 Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab200631)
- Anti-Human Kappa Chain antibody [EPR5539-71-8] - BSA and Azide free (ab211641)
- Rabbit monoclonal [EPR5539-71-8] Anti-Human kappa light chain (Alexa Fluor® 647) (ab236479)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab201490 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ELISA |
Use a concentration of 0.01 - 10 µg/ml.
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Notes |
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IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ELISA
Use a concentration of 0.01 - 10 µg/ml. |
Target
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Relevance
Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 3514 Human
- Omim: 147200 Human
- SwissProt: P01834 Human
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Alternative names
- HCAK 1 antibody
- HCAK1 antibody
- Ig kappa chain C region antibody
see all
Images
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Ab201490 was tested using a sandwich ELISA approach. The wells were coated with ab195576 at 0.5µg/ml at 50µl/well overnight at 4°C, followed by a 5% BSA blocking step for 2h RT. Human IgG (ab98981) was then added starting at 40 µg/ml and plasma/serum at 1:50 and gradually diluted 1:4, 50µl/well for 2h. Ab20149 was then added at 1:10,000 dilution, 50µl/well for 2h. A HRP-streptavidin (ab7403) was used at 1:10,000 dilution for 1h.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab201490)
IHC image of human kappa chain staining in a section of formalin-fixed paraffin-embedded normal human tonsil*, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab201490, at 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Staining corresponds to B cells expressing IgG commonly found in lymphoid follicles of human tonsil.
The inset background control image is taken from an identical assay without added antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab201490)
Negative IHC image of human kappa chain staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex*, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab201490, at 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Staining corresponds to immunoglobulins found in serum. As expected, staining diminishes further into the brain tissue due to the action of the blood-brain barrier.
The inset background control image is taken from an identical assay without added antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab201490 has not yet been referenced specifically in any publications.