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    biotin-human-kappa-chain-antibody-epr5539-71-8-ab201490.pdf

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Immunology Immunoglobulins Light Chain Kappa
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RecombinantRabMAb

Recombinant Biotin Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab201490)

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ELISA - Biotin Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab201490)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab201490)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab201490)
  • Biotin Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab201490)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Biotin Rabbit monoclonal [EPR5539-71-8] to Human Kappa Chain
  • Suitable for: IHC-P, ELISA
  • Reacts with: Human
  • Conjugation: Biotin

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Overview

  • Product name

    Biotin Anti-Human Kappa Chain antibody [EPR5539-71-8]
    See all Human Kappa Chain primary antibodies
  • Description

    Biotin Rabbit monoclonal [EPR5539-71-8] to Human Kappa Chain
  • Host species

    Rabbit
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Other Immunogen Type corresponding to Human Kappa Chain.

  • Positive control

    • IHC-P: Human normal tonsil tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol, 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5539-71-8
  • Isotype

    IgG
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Immunoglobulins
    • Light Chain
    • Kappa

Associated products

  • Alternative Versions

    • Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)
    • Alexa Fluor® 647 Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab200631)
    • Anti-Human Kappa Chain antibody [EPR5539-71-8] - BSA and Azide free (ab211641)
    • Rabbit monoclonal [EPR5539-71-8] Anti-Human kappa light chain (Alexa Fluor® 647) (ab236479)
  • Isotype control

    • Biotin Rabbit IgG - Isotype Control (ab200208)

Applications

Our Abpromise guarantee covers the use of ab201490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA Use a concentration of 0.01 - 10 µg/ml.

Target

  • Relevance

    Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin.
  • Cellular localization

    Cytoplasmic
  • Database links

    • Entrez Gene: 3514 Human
    • Omim: 147200 Human
    • SwissProt: P01834 Human
    • Unigene: 449621 Human
    • Alternative names

      • HCAK 1 antibody
      • HCAK1 antibody
      • Ig kappa chain C region antibody
      • IGKC antibody
      • IMMUNOGLOBULIN InV antibody
      • Immunoglobulin kappa constant antibody
      • Immunoglobulin kappa constant region antibody
      • Immunoglobulin kappa light chain antibody
      • KAPPA-CHAIN DEFICIENCY antibody
      • Km antibody
      • MGC11157 antibody
      • MGC62011 antibody
      • MGC72072 antibody
      • MGC88770 antibody
      • MGC88771 antibody
      • MGC88809 antibody
      see all

    Images

    • ELISA - Biotin Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab201490)
      ELISA - Biotin Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab201490)

      Ab201490 was tested using a sandwich ELISA approach. The wells were coated with ab195576 at 0.5µg/ml at 50µl/well overnight at 4°C, followed by a 5% BSA blocking step for 2h RT. Human IgG (ab98981) was then added starting at 40 µg/ml and plasma/serum at 1:50 and gradually diluted 1:4, 50µl/well for 2h. Ab20149 was then added at 1:10,000 dilution, 50µl/well for 2h. A HRP-streptavidin (ab7403) was used at 1:10,000 dilution for 1h.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab201490)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab201490)

      IHC image of human kappa chain staining in a section of formalin-fixed paraffin-embedded normal human tonsil*, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab201490, at 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      Staining corresponds to B cells expressing IgG commonly found in lymphoid follicles of human tonsil.

      The inset background control image is taken from an identical assay without added antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab201490)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab201490)

      Negative IHC image of human kappa chain staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex*, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab201490, at 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      Staining corresponds to immunoglobulins found in serum. As expected, staining diminishes further into the brain tissue due to the action of the blood-brain barrier.

      The inset background control image is taken from an identical assay without added antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Biotin Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab201490)
      Biotin Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab201490)

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab201490? Please let us know so that we can cite the reference in this datasheet.

    ab201490 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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