Biotin Anti-Human Serum Albumin antibody [AL-01] (ab81426)
![Western blot - Biotin Anti-Human Serum Albumin antibody [AL-01] (ab81426)](https://www.abcam.com/ps/products/81/ab81426/Images/ab81426-395137-AntiHuman-Serum-Albumin-antibody-AL01-Biotin-western-blot-plasma-human.jpg "Western blot - Biotin Anti-Human Serum Albumin antibody [AL-01] (ab81426)")
Key features and details
- Biotin Mouse monoclonal [AL-01] to Human Serum Albumin
- Suitable for: WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Overview
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Product name
Biotin Anti-Human Serum Albumin antibody [AL-01]
See all Human Serum Albumin primary antibodies -
Description
Biotin Mouse monoclonal [AL-01] to Human Serum Albumin -
Host species
Mouse -
Conjugation
Biotin -
Specificity
Reacts only with Human Serum Albumin. No cross-reactivity was observed with other serum proteins. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Does not react with: Mammals -
Immunogen
Fraction of proteins containing Albumin after ammonnium sulphate precipitation and DEAE-chromatography of Human serum.
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Positive control
- WB: Human plasma.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
AL-01 -
Isotype
IgG1 -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab81426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 69 kDa. |
Target
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Function
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. -
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Post-translational
modificationsKenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 213 Human
- Omim: 103600 Human
- SwissProt: P02768 Human
- Unigene: 418167 Human
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Alternative names
- alb antibody
- ALBU_HUMAN antibody
- Albumin (32 AA) antibody
see all
Images
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Western blot - Biotin Anti-Human Serum Albumin antibody [AL-01] (ab81426)All lanes : Biotin Anti-Human Serum Albumin antibody [AL-01] (ab81426) at 5 µg/ml
Lane 1 : Human plasma (non-reducing conditions)
Lane 2 : Human plasma (reducing conditions)
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate (reducing conditions)
Secondary
All lanes : IRDye800-conjugated Streptavidin
Predicted band size: 69 kDaThe experiment was conducted under reducing and non-reducing conditions.Negative control: HeLa cell lysate.
References (1)
ab81426 has been referenced in 1 publication.
- Edwards W et al. Targeting the ion channel Kv1.3 with scorpion venom peptides engineered for potency, selectivity, and half-life. J Biol Chem 289:22704-14 (2014). ELISA . PubMed: 24939846
