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    biotin-icam1-antibody-ab7815.pdf

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Immunology Cell Type Markers CD Adhesion
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Biotin Anti-ICAM1 antibody (ab7815)

  • Datasheet
Reviews (3)Q&A (10)References (6)

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Sandwich ELISA - Biotin Anti-ICAM1 antibody (ab7815)

    Key features and details

    • Biotin Rabbit polyclonal to ICAM1
    • Suitable for: Sandwich ELISA
    • Reacts with: Human, Chimpanzee, Baboon, Cynomolgus monkey, Rhesus monkey
    • Conjugation: Biotin
    • Isotype: IgG

    You may also be interested in

    Protein
    Recombinant Human ICAM1 protein (ab151393)
    Conjugation
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    Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
    Primary
    Product image
    Anti-ICAM1 antibody [YN1/1.7.4] (ab25375)

    View more associated products

    Overview

    • Product name

      Biotin Anti-ICAM1 antibody
      See all ICAM1 primary antibodies
    • Description

      Biotin Rabbit polyclonal to ICAM1
    • Host species

      Rabbit
    • Conjugation

      Biotin
    • Tested applications

      Suitable for: Sandwich ELISAmore details
    • Species reactivity

      Reacts with: Human, Chimpanzee, Baboon, Cynomolgus monkey, Rhesus monkey
    • Immunogen

      Full length protein (Human).

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.065% Sodium azide
      Constituent: 0.1% BSA
    • Concentration information loading...
    • Purification notes

      0.2 µm filtered
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Immunology
      • Cell Type Markers
      • CD
      • Adhesion
      • Cardiovascular
      • Atherosclerosis
      • Vascular Inflammation
      • Leukocyte recruitment
      • Cell adhesion molecules
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • B Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage
      • Immunology
      • Adaptive Immunity
      • Regulatory T Cells
      • Cardiovascular
      • Angiogenesis
      • Endothelial Cell Markers

    Associated products

    • Isotype control

      • Biotin Rabbit IgG - Isotype Control (ab200208)
    • Recombinant Protein

      • Recombinant Human ICAM1 protein (ab151393)
    • sELISA pair antibody

      • Anti-ICAM1 antibody [MEM-111] (ab2213)

    Applications

    Our Abpromise guarantee covers the use of ab7815 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [MEM-111] to ICAM1 (ab2213).

    For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab2213 as Capture.

    Target

    • Function

      ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
    • Sequence similarities

      Belongs to the immunoglobulin superfamily. ICAM family.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    • Post-translational
      modifications

      Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P05362 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3383 Human
      • Omim: 147840 Human
      • SwissProt: P05362 Human
      • Unigene: 643447 Human
      • Alternative names

        • Antigen identified by monoclonal antibody BB2 antibody
        • BB 2 antibody
        • BB2 antibody
        • CD 54 antibody
        • CD_antigen=CD54 antibody
        • CD54 antibody
        • Cell surface glycoprotein P3.58 antibody
        • Human rhinovirus receptor antibody
        • ICAM 1 antibody
        • ICAM-1 antibody
        • ICAM1 antibody
        • ICAM1_HUMAN antibody
        • intercellular adhesion molecule 1 (CD54) antibody
        • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
        • Intercellular adhesion molecule 1 antibody
        • Major group rhinovirus receptor antibody
        • MALA 2 antibody
        • MALA2 antibody
        • MyD 10 antibody
        • MyD10 antibody
        • P3.58 antibody
        • Surface antigen of activated B cells antibody
        • Surface antigen of activated B cells, BB2 antibody
        see all

      Images

      • Sandwich ELISA - Biotin Anti-ICAM1 antibody (ab7815)
        Sandwich ELISA - Biotin Anti-ICAM1 antibody (ab7815)
        Standard Curve for ICAM1 (Analyte: ab82125) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [MEM-111] to ICAM1 (ab2213) at 5ug/ml and Detector Antibody Rabbit polyclonal to ICAM1 (Biotin) (ab7815) at 0.5ug/ml

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (6)

      Publishing research using ab7815? Please let us know so that we can cite the reference in this datasheet.

      ab7815 has been referenced in 6 publications.

      • Zhang Z  et al. Antioxidant and anti-inflammatory effects of DHK-medicated serum on high glucose-induced injury in endothelial cells. Mol Med Rep 16:7745-7751 (2017). PubMed: 28944884
      • Zhang Q  et al. Toll-like receptor 4 contributes to acute kidney injury after cardiopulmonary resuscitation in mice. Mol Med Rep 14:2983-90 (2016). Mouse . PubMed: 27510583
      • Malik IA  et al. Role of PECAM-1 in radiation-induced liver inflammation. J Cell Mol Med 19:2441-52 (2015). PubMed: 26177067
      • Rohlena J  et al. Endothelial CD81 is a marker of early human atherosclerotic plaques and facilitates monocyte adhesion. Cardiovasc Res 81:187-96 (2009). Electron Microscopy ; Human . PubMed: 18805782
      • Fransson J  et al. Rapid induction of apoptosis in B-cell lymphoma by functionally isolated human antibodies. Int J Cancer 119:349-58 (2006). Blocking ; Human . PubMed: 16477633
      • Ohwada A  et al. Acid exposure potentiates intercellular adhesion molecule-1 and e-cadherin expression on A549 alveolar lining epithelial cells. Exp Lung Res 29:389-400 (2003). ICC/IF ; Human . PubMed: 12888451

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-10 of 13 Abreviews or Q&A

      Flow Cytometry abreview for Anti-ICAM1 antibody (Biotin)

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Rhesus monkey Cell (choroidal endothelial cells)
      Permeabilization
      No
      Gating Strategy
      size and granularity
      Specification
      choroidal endothelial cells
      Preparation
      Cell harvesting/tissue preparation method: cells were detached from tissue culture plates with Accutase
      Sample buffer: 2mM EDTA + 0.5% BSA in PBS
      Fixation
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 14 2017

      Western blot abreview for Anti-ICAM1 antibody (Biotin)

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      50 µg
      Gel Running Conditions
      Reduced Denaturing (12%)
      Sample
      Mouse Tissue lysate - whole (Liver)
      Specification
      Liver
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 27 2013

      Question

      Ab7815 non conjugé?

      Read More

      Abcam community

      Verified customer

      Asked on Apr 17 2013

      Answer

      Apres plusieurs conversations avec notre service commercial j’ai réussi d’ajouter l’anticorps ab7815 non conjugué au catalogue. La référence est ab170497.

      Read More

      Abcam Scientific Support

      Answered on Apr 17 2013

      Question

      We have informed that the product ab27536, which was discontinued. We have been received the information given by you, but I contacted with the customer and she told me she need the antibody is able to recognize the ICAM1 protein intra- and extracellular in samples extracted from human. I would like you may confirm these antibodies recommended by you could work in this condition. Additionally, in the Abcam webpage, I found three antibodies, which are shown below: Anti-ICAM1 antibody [MEM-111] ab2213 Anti-ICAM1 antibody [YN1/1.7.4] ab25375 Anti-ICAM1 antibody (Biotin) ab7815 Which could these ones the differences with the others? Thanks for your support

      Read More

      Abcam community

      Verified customer

      Asked on Jun 05 2012

      Answer

      Thank you for the response.

      Any monoclonal antibody is produced by a single immune cell, which has been cloned. These will only recognize a single epitope of the target protein. This epitope typically is 5-10 amino acids in length. Therefore any single monoclonal antibody will only recognize a region within a single domain and not stain both the intracellular and extracellular region of the target protein.

      Polyclonal antibodies are created by many different immune cells and will contain multiple epitope recognition sites. Although the amount of antibody specific to a particular epitope will vary from lot-to-lot, it is possible for a polyclonal to therefore contain antibodies which will recognize different domains.

      I have reviewed the antibodies which you have listed as well as other polyclonals in our catalog to try and find a product which might be able to recognize the intracellular and extracellular regions.

      ab2213Anti-ICAM1 antibody [MEM-111]: This monoclonal antibody was created with an immunogen from a whole tissue / cell preparation (Human). We have not mapped the exact location of the epitope. Therefore the epitope recognized might be intracellular, transmembrane or extracellular.

      ab25375Anti-ICAM1 antibody [YN1/1.7.4]: This product is also monoclonal.The details of the immunogen for this antibody are not available. However as a monoclonal, this product will not be able to recognize sites in multiple domains.

      ab7815Anti-ICAM1 antibody (Biotin): This product is a rabbit polyclonal and has been raised against the whole protein. As such it is possible that the product will contain antibodies against both extracellular and intracellular regions. We do not test for this type of response and cannot guarantee that the productwill recognizeboth domains. Please be aware that this product is also biotin labeled.

      Our other rabbit polyclonal antibodies were each created from recombinant fragments and would also not be able to recognize both extracellular and intracellular domains.

      I hope this information is helpful. Please do not hesitate to contact me with further questions.

      Read More

      Abcam Scientific Support

      Answered on Jun 05 2012

      Question

      Dear Sir/Madam

      I would like to ask if antibodies with cat no ab7815 and ab33451 are able to use for IHC staining in paraffin embedded tissue.

      Thank you very much.

      Regards,
      Kay

      Read More

      Abcam community

      Verified customer

      Asked on May 03 2012

      Answer

      Thank you for contacting us.

      ab7815 and ab33451 have been tested with Frozen sections only and we do not have any empirical data to support their performance with paraffin tissues.

      I have found the following ICAM1 and Monocyte + macrophage antibodies which have been tested in IHC-P and might be of your interest.

      Anti-ICAM1 antibody:

      ab2213 ab109361 ab33894. They are not biotin labelled.

      Anti-Monocyte + Macrophage antibody


      ab51934. React with human.



      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      Answered on May 03 2012

      Question

      I have a reply from the customer. Regarding the IHC testing, I was under the impression that they used paraffin sections, however, they used sections that were slightly fixed, impregnated with Sucrose and frozen to cut on a freezing microtome. Can you please take a look at the IHC form again with this information in mind? Regarding the suggestions, they do not have a skin biopsy available for a positive control. They did buy the antibody for IHC and only tried it in WB after it did not work in IHC, so they do not want to try the WB again if they do not have to.



      Do you have suggestions for the antibody in IHC?

      Thank you,

      Read More

      Abcam community

      Verified customer

      Asked on Feb 02 2012

      Answer

      Thank you for your email.

      I have a couple recommendation for your customer when using IHC-Fr.

      1.) I strongly suggest to run a positive control. ICAM1 belongs to the homing receptors and to my knowledge should not be expressed in brain. These homing receptor are expressed in site with inflammation to accumulate lymphocytes. If treated skin biopsies are not available, lung or bone marrow might be suitable.

      2.) According to the protocol your customer send us, the mouse brain is fixed twice: perfusion fixed and fixed for 4days after. This strong (double-)fixation can lead to confirmation changes in the target protein which renders the antibody unable to recognize the target protein.
      I recommend to try various antigen retrieval methods. This has to be tested experimentally.

      3.) Your customer is correct, this antibody is labeled with biotin. Without using a secondary antibody there is less amplification of the signal and I therefore suggest to dilute the antibody less and to try 1:50.

      I hope these tips can help improve the result. Please do not hesitate to contact me again with any further questions.

      Read More

      Abcam Scientific Support

      Answered on Feb 02 2012

      Question


      Good morning,

      I had a customer contact me regarding some problems with AB7815 in WB and IHC (no signal). I realize that this antibody is not longer validated for paraffin sections, but the customer also tried it in WB without success. This item was ordered on PO xxxx. Can you please take a look at the customer's protocols attached?



      Thank you



      Kind regards

      Read More

      Abcam community

      Verified customer

      Asked on Jan 31 2012

      Answer

      Thank you and your customer for taking the time to complete our questionnaire and contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from this antibody. I can confirm that ab7815 is covered by our Abpromise guarantee when used in WB.

      The details your customer has kindly provided will enable us to investigate this case for your customer and this is also helpful in our records for monitoring of quality.

      Reviewing this case, I would like to offer some suggestions to help optimise the results from ab7815:

      1.) I suggest use a positive control: Fresh skin biopsy (small fresh skin biopsy incubated for 4 hours with TNF (1 ng/ml), IL-1 (100 u/ml) or LPS (1 ìg/ml) in tissue culture medium at 37oC).

      2.) I recommend to use reducing and denaturing conditions: add SDS to the lysis puffer and add beta mercaptho-ethanol to the loading buffer.

      3.) Blocking in BSA can significantly increase the signal of some antibodies. Therefore I suggest to use 5% BSA as blocking agent.

      4.) Since there is no background I suggest to not use any blocking agent in the antibody solution and to dilute the antibody less. An other customer had good results with a dilution of 1:500.

      We are happy to offer this technical support. In the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

      I hope this information is helpful, thank you and your customer for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

      Read More

      Abcam Scientific Support

      Answered on Jan 31 2012

      Question

      Muchas gracias, cuando sepas algo nos comentas, y elegimos el que creamos pueda ser mejor.

      Read More

      Abcam community

      Verified customer

      Asked on Jan 25 2012

      Answer



      I can confirm that we didn't test the antibody (ab106777: Anti-VCAM1) in Rabbit tissue, as soon as we have further information we will update our on-line datasheet accordingly.

      Read More

      Abcam Scientific Support

      Answered on Jan 25 2012

      Question

      Muchas gracias, cuando sepas algo nos comentas, y elegimos el que creamos pueda ser mejor.

      Read More

      Abcam community

      Verified customer

      Asked on Jan 25 2012

      Answer

      Thank you for your interest.



      The reactivity of this antibody (ab2213) with rabbit samples on Western blot application is probable but it has not been tested yet.



      Hope this will be useful for you!

      Read More

      Abcam Scientific Support

      Answered on Jan 25 2012

      Western blot abreview for Anti-ICAM1 antibody (Biotin)

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (brain)
      Loading amount
      40 µg
      Specification
      brain
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Dr. Alicia Moreno

      Verified customer

      Submitted Sep 06 2010

      1-10 of 13 Abreviews or Q&A

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