Biotin Anti-ICAM1 antibody (ab7815)

Apres plusieurs conversations avec notre service commercial j’ai réussi d’ajouter l’anticorps ab7815 non conjugué au catalogue. La référence est ab170497.

Thank you for the response.

Any monoclonal antibody is produced by a single immune cell, which has been cloned. These will only recognize a single epitope of the target protein. This epitope typically is 5-10 amino acids in length. Therefore any single monoclonal antibody will only recognize a region within a single domain and not stain both the intracellular and extracellular region of the target protein.

Polyclonal antibodies are created by many different immune cells and will contain multiple epitope recognition sites. Although the amount of antibody specific to a particular epitope will vary from lot-to-lot, it is possible for a polyclonal to therefore contain antibodies which will recognize different domains.

I have reviewed the antibodies which you have listed as well as other polyclonals in our catalog to try and find a product which might be able to recognize the intracellular and extracellular regions.

ab2213Anti-ICAM1 antibody [MEM-111]: This monoclonal antibody was created with an immunogen from a whole tissue / cell preparation (Human). We have not mapped the exact location of the epitope. Therefore the epitope recognized might be intracellular, transmembrane or extracellular.

ab25375Anti-ICAM1 antibody [YN1/1.7.4]: This product is also monoclonal.The details of the immunogen for this antibody are not available. However as a monoclonal, this product will not be able to recognize sites in multiple domains.

ab7815Anti-ICAM1 antibody (Biotin): This product is a rabbit polyclonal and has been raised against the whole protein. As such it is possible that the product will contain antibodies against both extracellular and intracellular regions. We do not test for this type of response and cannot guarantee that the productwill recognizeboth domains. Please be aware that this product is also biotin labeled.

Our other rabbit polyclonal antibodies were each created from recombinant fragments and would also not be able to recognize both extracellular and intracellular domains.

I hope this information is helpful. Please do not hesitate to contact me with further questions.

Thank you for contacting us.

ab7815 and ab33451 have been tested with Frozen sections only and we do not have any empirical data to support their performance with paraffin tissues.

I have found the following ICAM1 and Monocyte + macrophage antibodies which have been tested in IHC-P and might be of your interest.

Anti-ICAM1 antibody:

ab2213 ab109361 ab33894. They are not biotin labelled.

Anti-Monocyte + Macrophage antibody


ab51934. React with human.



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for your email.

I have a couple recommendation for your customer when using IHC-Fr.

1.) I strongly suggest to run a positive control. ICAM1 belongs to the homing receptors and to my knowledge should not be expressed in brain. These homing receptor are expressed in site with inflammation to accumulate lymphocytes. If treated skin biopsies are not available, lung or bone marrow might be suitable.

2.) According to the protocol your customer send us, the mouse brain is fixed twice: perfusion fixed and fixed for 4days after. This strong (double-)fixation can lead to confirmation changes in the target protein which renders the antibody unable to recognize the target protein.
I recommend to try various antigen retrieval methods. This has to be tested experimentally.

3.) Your customer is correct, this antibody is labeled with biotin. Without using a secondary antibody there is less amplification of the signal and I therefore suggest to dilute the antibody less and to try 1:50.

I hope these tips can help improve the result. Please do not hesitate to contact me again with any further questions.

Thank you and your customer for taking the time to complete our questionnaire and contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from this antibody. I can confirm that ab7815 is covered by our Abpromise guarantee when used in WB.

The details your customer has kindly provided will enable us to investigate this case for your customer and this is also helpful in our records for monitoring of quality.

Reviewing this case, I would like to offer some suggestions to help optimise the results from ab7815:

1.) I suggest use a positive control: Fresh skin biopsy (small fresh skin biopsy incubated for 4 hours with TNF (1 ng/ml), IL-1 (100 u/ml) or LPS (1 ìg/ml) in tissue culture medium at 37oC).

2.) I recommend to use reducing and denaturing conditions: add SDS to the lysis puffer and add beta mercaptho-ethanol to the loading buffer.

3.) Blocking in BSA can significantly increase the signal of some antibodies. Therefore I suggest to use 5% BSA as blocking agent.

4.) Since there is no background I suggest to not use any blocking agent in the antibody solution and to dilute the antibody less. An other customer had good results with a dilution of 1:500.

We are happy to offer this technical support. In the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I hope this information is helpful, thank you and your customer for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

I can confirm that we didn't test the antibody (ab106777: Anti-VCAM1) in Rabbit tissue, as soon as we have further information we will update our on-line datasheet accordingly.

Thank you for your interest.



The reactivity of this antibody (ab2213) with rabbit samples on Western blot application is probable but it has not been tested yet.



Hope this will be useful for you!