Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Biotin Rabbit monoclonal [RM123] to IgD
- Suitable for: Sandwich ELISA, ELISA, IHC
- Reacts with: Human
- Conjugation: Biotin
Product nameBiotin Anti-IgD antibody [RM123]
See all IgD primary antibodies
DescriptionBiotin Rabbit monoclonal [RM123] to IgD
Specificityab224182 reacts to human IgD. No cross reactivity with human IgG, IgM, IgA, or IgE.
Tested applicationsSuitable for: Sandwich ELISA, ELISA, IHCmore details
Species reactivityReacts with: Human
Full length native protein (purified) corresponding to Human IgD aa 1-384.
APTKAPDVFPIISGCRHPKDNSPVVLACLITGYHPTSVTVTWYMGTQSQP QRTFPEIQRRDSYYMTSSQLSTPLQQWRQGEYKCVVQHTASKSKKEIFRW PESPKAQASSVPTAQPQAEGSLAKATTAPATTRNTGRGGEEKKKEKEKEE QEERETKTPECPSHTQPLGVYLLTPAVQDLWLRDKATFTCFVVGSDLKDA HLTWEVAGKVPTGGVEEGLLERHSNGSQSQHSRLTLPRSLWNAGTSVTCT LNHPSLPPQRLMALREPAAQAPVKLSLNLLASSDPPEAASWLLCEVSGFS PPNILLMWLEDQREVNTSGFAPARPPPQPGSTTFWAWSVLRVPAPPSPQP ATYTCVVSHEDSRTLLNASRSLEVSYVTDHGPMK
Database link: P01880
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityProtein A purified
Purification notesab224182 was purified from an animal origin–free culture supernatant .
Our Abpromise guarantee covers the use of ab224182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.
Use: 25 - 200 ng/well (for capture); 0.01 - 0.1 μg/ml (for detection).
|ELISA||Use at an assay dependent concentration.|
|IHC||Use a concentration of 0.5 - 2 µg/ml.|
RelevanceLittle is known about the normal function of IgD, and few clinical signs or symptoms are associated with its absence. Individuals with low or absent levels of IgD do not appear unusually predisposed to infections. In mice, IgD may substitute for some functions of IgM when IgM is absent. IgD is expressed on peripheral B cells and is used as a pan B cell marker.
- Ig delta chain C region antibody
- IGHD antibody
- Immunoglobulin heavy constant delta antibody
Sandwich ELISA using ab224182 as the capture antibody (100 ng/well), and ab193185 as the detection antibody, followed by an alkaline phosphatase-conjugated streptavidin.
A titre ELISA using ab224182. The plate was coated with different amounts of human IgD from plasma. A serial dilution of ab224182 was used as primary antibody. An alkaline phosphatase-conjugated Anti-Rabbit IgG was used as secondary antibody.
ELISA of human immunoglobulins shows that ab224182 reacts to IgD from human plasma and IgDκ from human melanoma. No cross reactivity with human IgG, IgM, IgA or IgE. The plate was coated with 50 ng/well of the different immunoglobulins. 200 ng/ml, 50 ng/ml or 10 ng/ml of ab224182 was used as the primary antibody. An alkaline phosphatase-conjugated Anti-Rabbit IgG was used as secondary antibody.
Human lymphoid tissue stained for IgD with ab224182 (2 μg/ml) in immunohistochemical analysis.
ab224182 has not yet been referenced specifically in any publications.