Key features and details
- Biotin Rabbit polyclonal to IGFBP1
- Suitable for: WB, Sandwich ELISA
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
Product nameBiotin Anti-IGFBP1 antibody
See all IGFBP1 primary antibodies
DescriptionBiotin Rabbit polyclonal to IGFBP1
Tested applicationsSuitable for: WB, Sandwich ELISAmore details
Species reactivityReacts with: Recombinant fragment
Predicted to work with: Human
Recombinant full length protein corresponding to Human IGFBP1 aa 26-259. Produce in E. coli. Full length mature chain without signal peptide.
MAPWQCAPCSAEKLALCPPVSASCSEVTRSAGCGCCPMCALPLGAACGVA TARCARGLSCRALPGEQQPLHALTRGQGACVQESDASAPHAAEAGSPESP ESTEITEEELLDNFHLMAPSEEDHSILWDAISTYDGSKALHVTNIKKWKE PCRIELYRVVESLAKAQETSGEEISKFYLPNCNKNGFYHSRQCETSMDGE AGLCWCVYPWNGKRIPGSPEIRGDPNCQIYFNVQN
Database link: P08833
- WB: Recombinant human IGFBP1 protein. sELISA: Recombinant human IGFBP1 protein.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferConstituents: PBS, 0.1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab246315 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml.|
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml.|
FunctionIGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.
Sequence similaritiesContains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain.
modificationsPhosphorylated; probably by casein kinase II. Alters the affinity of the protein for IGFs.
- Information by UniProt
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To detect human IGFBP1 by Western Blot analysis ab246315 can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human IGFBP1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane 1: Marker.
Lanes 2-12: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng/lane respectively.
To detect human IGFBP1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab246315 is required. This biotinylated polyclonal antibody, in conjunction with a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human IGFBP1.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab246315 has not yet been referenced specifically in any publications.