Recombinant

Recombinant Biotin Anti-IGLC1 antibody [RM127] (ab224180)

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ELISA - Biotin Anti-IGLC1 antibody [RM127] (ab224180)
  • ELISA - Biotin Anti-IGLC1 antibody [RM127] (ab224180)
  • Immunohistochemistry - Biotin Anti-IGLC1 antibody [RM127] (ab224180)
  • Biotin Anti-IGLC1 antibody [RM127] (ab224180)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Biotin Rabbit monoclonal [RM127] to IGLC1
  • Suitable for: ELISA, IHC
  • Reacts with: Human
  • Conjugation: Biotin

Overview

  • Product name

    Biotin Anti-IGLC1 antibody [RM127]

  • Description

    Biotin Rabbit monoclonal [RM127] to IGLC1

  • Host species

    Rabbit

  • Conjugation

    Biotin

  • Specificity

    ab224180 reacts to the lambda light chain of human immunoglobulins.

  • Tested applications

    Suitable for: ELISA, IHCmore details

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat, Goat

  • Immunogen

    Full length native protein (purified) corresponding to Human IGLC1.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    ab224180 was purified from an animal origin–free culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    RM127

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab224180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.05 - 0.2 µg/ml.
IHC Use a concentration of 0.5 - 2 µg/ml.

Target

  • Cellular localization

    Extracellular region, plasma membrane.

  • Database links

    • Alternative names

      • Ig lambda-1 chain C regions antibody
      • IGLC antibody
      • immunoglobulin lambda constant 1 (Mcg marker) antibody
      • Immunoglobulin lambda constant region 1 antibody
      • Immunoglobulin: lambda light chain antibody
      see all

    Images

    • ELISA - Biotin Anti-IGLC1 antibody [RM127] (ab224180)
      ELISA - Biotin Anti-IGLC1 antibody [RM127] (ab224180)

      A titer ELISA using ab224180. The plate was coated with different amounts of human IgG3λ. A serial dilution of ab224180 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG was used as the secondary antibody.

    • ELISA - Biotin Anti-IGLC1 antibody [RM127] (ab224180)
      ELISA - Biotin Anti-IGLC1 antibody [RM127] (ab224180)

      ELISA of human immunoglobulins shows ab224180 reacts to the lambda light chain of human immunoglobulins. No cross reactivity with the kappa light chain, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL or 50 ng/mL of ab224180 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG was used as the secondary antibody.

    • Immunohistochemistry - Biotin Anti-IGLC1 antibody [RM127] (ab224180)
      Immunohistochemistry - Biotin Anti-IGLC1 antibody [RM127] (ab224180)

      Human tonsil tissue stained for IGLC1 using ab224180 at 2 µg/ml in immunohistochemical analysis.

    • Biotin Anti-IGLC1 antibody [RM127] (ab224180)
      Biotin Anti-IGLC1 antibody [RM127] (ab224180)

    References (0)

    Publishing research using ab224180? Please let us know so that we can cite the reference in this datasheet.

    ab224180 has not yet been referenced specifically in any publications.

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