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    biotin-il-12-antibody-ab83448.pdf

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Immunology Innate Immunity Cytokines Interleukins
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Biotin Anti-IL-12 antibody (ab83448)

  • Datasheet
Reviews (1)Q&A (1)

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Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Biotin Goat polyclonal to IL-12
  • Reacts with: Human
  • Conjugation: Biotin
  • Isotype: IgG

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Protein
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Recombinant human IL-12 p70 protein (Active) (ab259418)
ELISA
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Human IL-12 ELISA Kit High Sensitivity (ab46035)

View more associated products

Overview

  • Product name

    Biotin Anti-IL-12 antibody
    See all IL-12 primary antibodies
  • Description

    Biotin Goat polyclonal to IL-12
  • Host species

    Goat
  • Conjugation

    Biotin
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Horse, Baboon, Macaque monkey
  • Immunogen

    Recombinant full length protein corresponding to Human IL-12.

  • Positive control

    • Human IL-12
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Lyophilized:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 1.0 mg/ml.
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was purified by affinity chromatography and then biotinylated.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic inhibitory factors
    • Cancer
    • Tumor immunology
    • Cytokines
    • Interleukins

Associated products

  • Isotype control

    • Biotin Goat IgG - Isotype Control (ab37376)
  • Recombinant Protein

    • Recombinant human IL-12 p70 protein (Active) (ab259418)
    • Recombinant mouse IL-12 protein (Active) (ab259419)

Target

  • Function

    Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
  • Sequence similarities

    Belongs to the IL-6 superfamily.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P29459 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3592 Human
    • Entrez Gene: 3593 Human
    • Omim: 161560 Human
    • Omim: 161561 Human
    • SwissProt: P29459 Human
    • SwissProt: P29460 Human
    • Unigene: 673 Human
    • Alternative names

      • CLMF antibody
      • CLMF p35 antibody
      • CLMF p40 antibody
      • CLMF1 antibody
      • CLMF2 antibody
      • Cytotoxic lymphocyte maturation factor 1, p35 antibody
      • Cytotoxic lymphocyte maturation factor 2 antibody
      • Cytotoxic lymphocyte maturation factor 35 kDa subunit antibody
      • Cytotoxic lymphocyte maturation factor 40 kDa subunit antibody
      • IL 12A antibody
      • IL 2 subunit p40 antibody
      • IL-12 subunit p35 antibody
      • IL-12A antibody
      • IL-12B antibody
      • IL12 antibody
      • IL12, p35 subunit antibody
      • IL12, subunit p40 antibody
      • IL12A antibody
      • IL12A_HUMAN antibody
      • IL12B antibody
      • IL35 subunit antibody
      • Interleukin 12 alpha chain antibody
      • Interleukin 12 beta chain antibody
      • Interleukin 12 subunit beta antibody
      • Interleukin 12, p40 antibody
      • Interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) antibody
      • Interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) antibody
      • Interleukin 12B antibody
      • Interleukin-12 subunit alpha antibody
      • Natural killer cell stimulatory factor 1, 35 kD subunit antibody
      • natural killer cell stimulatory factor 2 antibody
      • Natural killer cell stimulatory factor, 40 kD subunit antibody
      • NF cell stimulatory factor chain 1 antibody
      • NFSK antibody
      • NK cell stimulatory factor chain 1 antibody
      • NK cell stimulatory factor chain 2 antibody
      • NKSF antibody
      • NKSF1 antibody
      • NKSF2 antibody
      • P35 antibody
      see all

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab83448? Please let us know so that we can cite the reference in this datasheet.

    ab83448 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews or Q&A

    ELISA abreview for Anti-IL-12 antibody (Biotin)

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Horse Recombinant protein (IL-12)
    Specification
    IL-12
    Type
    Sandwich (Detection)
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 05 2015

    Question

    where is biotin located?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 14 2014

    Answer

    Thank you for contacting us.

    During the chemical conjugation process, biotin is non-specifically attached to free groups on the antibody and multiple biotins will conjugate to a single antibody. For this reason, there is no single site at which biotin is attached to this antibody.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information

    Read More

    Heather Allen

    Abcam Scientific Support

    Answered on Apr 15 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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