Key features and details
- Biotin Rabbit polyclonal to IL-15
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Product nameBiotin Anti-IL-15 antibody
See all IL-15 primary antibodies
DescriptionBiotin Rabbit polyclonal to IL-15
Tested applicationsSuitable for: Sandwich ELISA, WBmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human IL-15.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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FormLyophilized:Centrifuge vial prior to opening. Reconstitute in 50ul sterile PBS containing 0.1% BSA
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituents: 0.1% BSA, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab83157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml.
Detection limit for recombinant Human protein: 0.2 - 0.4 ng/well, using 100 µl/well antibody solution.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 18 kDa.
Detection limit for recombinant Human protein: 1.5 - 3.0 ng/lane, under reducing or non-reducing conditions.
FunctionCytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha.
Tissue specificityMost abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15-S21AA is preferentially expressed in tissues such as testis and thymus.
Sequence similaritiesBelongs to the IL-15/IL-21 family.
Cellular localizationSecreted and Cytoplasm. Nucleus. IL15-S21AA is not secreted, but rather is stored intracellularly, appearing in the nucleus and cytoplasmic components.
- Information by UniProt
- IL 15 antibody
- IL-15 antibody
- IL15 antibody
To detect human IL-15 by Western Blot analysis ab83157 can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human IL-15 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Image shown is reducing conditions; Human recombinant IL-15 protein at 250 - 0.24 ng/ml.
Lane 1 is MW marker, lanes 2-12 human recombinant IL-15 protein.
To detect human IL-15 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab83157 is required. This biotinylated polyclonal antibody, in conjunction with anti-IL-15 (ab9581) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human IL-15.
ab83157 has not yet been referenced specifically in any publications.