Biotin Anti-IL-15 antibody (ab83157)
Key features and details
- Biotin Rabbit polyclonal to IL-15
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-IL-15 antibody
See all IL-15 primary antibodies -
Description
Biotin Rabbit polyclonal to IL-15 -
Host species
Rabbit -
Conjugation
Biotin -
Tested applications
Suitable for: Sandwich ELISA, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human IL-15.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Lyophilized:Centrifuge vial prior to opening. Reconstitute in 50ul sterile PBS containing 0.1% BSA -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 0.1% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab83157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use a concentration of 0.25 - 1 µg/ml. Detection limit for recombinant Human protein: 0.2 - 0.4 ng/well, using 100 µl/well antibody solution. |
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| WB | Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 18 kDa. Detection limit for recombinant Human protein: 1.5 - 3.0 ng/lane, under reducing or non-reducing conditions. |
Target
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Function
Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha. -
Tissue specificity
Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15-S21AA is preferentially expressed in tissues such as testis and thymus. -
Sequence similarities
Belongs to the IL-15/IL-21 family. -
Cellular localization
Secreted and Cytoplasm. Nucleus. IL15-S21AA is not secreted, but rather is stored intracellularly, appearing in the nucleus and cytoplasmic components. - Information by UniProt
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Database links
- Entrez Gene: 3600 Human
- Omim: 600554 Human
- SwissProt: P40933 Human
- Unigene: 654378 Human
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Alternative names
- IL 15 antibody
- IL-15 antibody
- IL15 antibody
see all
Images
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Western blot - Biotin Anti-IL-15 antibody (ab83157)
To detect human IL-15 by Western Blot analysis ab83157 can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human IL-15 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Image shown is reducing conditions; Human recombinant IL-15 protein at 250 - 0.24 ng/ml.
Lane 1 is MW marker, lanes 2-12 human recombinant IL-15 protein.
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Sandwich ELISA - Biotin Anti-IL-15 antibody (ab83157)To detect human IL-15 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab83157 is required. This biotinylated polyclonal antibody, in conjunction with anti-IL-15 (ab9581) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human IL-15.
Datasheets and documents
References (0)
ab83157 has not yet been referenced specifically in any publications.
