Biotin Anti-IL-4 antibody (ab84278)
Key features and details
- Biotin Rabbit polyclonal to IL-4
- Suitable for: Sandwich ELISA, WB
- Reacts with: Rat
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-IL-4 antibody
See all IL-4 primary antibodies -
Description
Biotin Rabbit polyclonal to IL-4 -
Host species
Rabbit -
Conjugation
Biotin -
Tested applications
Suitable for: Sandwich ELISA, WBmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Recombinant full length protein corresponding to Rat IL-4.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Lyophilized:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purification notes
ab84278 was purified by affinity chromatography and then biotinylated. It is sterile filtered. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab84278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use a concentration of 0.25 - 1 µg/ml. Use 100 μl/well antibody solution. Detects at least 0.2 – 0.4 ng/well of recombinant rat IL-4. |
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| WB | Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 16 kDa. Detection limit for recombinant rat IL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Target
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Function
Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. -
Involvement in disease
Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. -
Sequence similarities
Belongs to the IL-4/IL-13 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 287287 Rat
- SwissProt: P20096 Rat
- Unigene: 108255 Rat
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Alternative names
- B cell growth factor 1 antibody
- B cell IgG differentiation factor antibody
- B Cell Stimulatory Factor 1 antibody
see all
Images
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Western blot - Biotin Anti-IL-4 antibody (ab84278)
To detect rat IL-4 by Western Blot analysis ab84278 can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant rat IL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Image shown is non-reducing conditions; Rat recombinant IL-4 protein at 250 - 0.24 ng/ml.
Lane 1 is MW marker, lanes 2-12 rat recombinant IL-4 protein.
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Sandwich ELISA - Biotin Anti-IL-4 antibody (ab84278)To detect rat IL-4 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab84278 is required. Using this antibody in conjunction with an anti-IL-4 (ab9811) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant rat IL-4.
Datasheets and documents
References (0)
ab84278 has not yet been referenced specifically in any publications.
