Biotin Anti-IL-6 antibody (ab84251)
Key features and details
- Biotin Rabbit polyclonal to IL-6
- Suitable for: WB, Sandwich ELISA
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-IL-6 antibody
See all IL-6 primary antibodies -
Description
Biotin Rabbit polyclonal to IL-6 -
Host species
Rabbit -
Conjugation
Biotin -
Tested applications
Suitable for: WB, Sandwich ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human IL-6.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purification notes
ab84251 was purified by affinity chromatography and then biotinylated.Filter sterilised. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ELISA pair antibody
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration.
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| Sandwich ELISA |
Use at an assay dependent concentration.
To detect hIL-6 by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.25 – 1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Mouse monoclonal to IL6 (ab9324) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIL-6. |
| Notes |
|---|
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WB
Use at an assay dependent concentration. |
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Sandwich ELISA
Use at an assay dependent concentration. To detect hIL-6 by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.25 – 1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Mouse monoclonal to IL6 (ab9324) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIL-6. |
Target
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Function
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. -
Involvement in disease
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3569 Human
- Omim: 147620 Human
- SwissProt: P05231 Human
- Unigene: 654458 Human
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Alternative names
- Interleukin BSF 2 antibody
- B cell differentiation factor antibody
- B cell stimulatory factor 2 antibody
see all
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab84251 has not yet been referenced specifically in any publications.