Biotin Anti-Insulin + Proinsulin antibody [D3E7] (ab20756)

This antibody is already conjugated to biotin and therefore does not need a secondary antibody for detection.
What you will need is avidin or streptavidin conjugated to an enzyme or fluorochrome that will bind to multiple sites on the biotinylated primary antibody. We have several streptavidin proteins in our catalogue conjugated to an enzyme or fluorochrome. The conjugate you choose will depend on your detection system.
For more information, please visit:
https://www.abcam.com/index.html?pageconfig=resource&rid=12922
https://www.abcam.com/index.html?pageconfig=resource&rid=12723
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Les anticorps utilisés dans le kit ELISA ab100578 sont les anticorps ab8304 comme anticorps de capture et ab20756 comme anticorps de détection. Ces deux anticorps sont testés et garantis chez le rat. Le kit lui-même n’a pas été testé chez le rat mais il y’a des chances que cela fonctionne. Si vous souhaitez le tester, je peux vous faire une offre de test.

Thank you for your inquiry. 
The antibody pair that we use in the Human Insulin ELISA Kit (ab100578) is capture antibody (ab8304) and detection antibody (ab20756). 
https://www.abcam.com/insulin--proinsulin-antibody-d6c4-ab8304.html
https://www.abcam.com/insulin-proinsulin-antibody-d3e7-biotin-ab20756.html
We have not specifically tested human analogs with this kit but based on sequence similarity, we would expect the kit to recognize these as well.  However, we wouldn't guarantee it since it hasn't been specifically tested.
I hope this information helps. Please contact us with any other questions.

Thank you for your further enquiry.

I am sorry to confirm that as far as we are aware, ab8304 and ab20756 insulin antibodies have never been tested in ELISPOT. All tested and guaranteed applications are specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

If you would like to test these antibodies in ELISPOT, please contactmeprior to the purchase by replying to this message as you may be eligible for our testing discount program.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact me for any further advice or information in this regard.

Thank you for your enquiry which has been forwarded to the scientific support team. We can be contacted at mailto:technical@abcam.com, or directly from the website.

I can confirm that ab8304 can be paired for Sandwich ELISA with Mouse monoclonal [D3E7] to Insulin + Proinsulin (Biotin) (ab20756).

In this case, it is ok to use a mouse monoclonal for the coating antibody and detection antibody, because the detection antibody is directly conjugated to biotin.

(Without the biotin, if you needed to use a conjugated secondary anti mouse antibody, then this would not work as the secondary would also detect the coating antibody, which is covering the whole plate).

I hope this information is helpful. If you have any further questions, please do not hesitate to contact us.

Thank you for contacting us. Yes, the clones D6C4 and D3E7 have been validated for use as a sandwich ELISA pair and will be guaranteed for that purpose. Because the detection antibody you are choosing is already biotinylated, it will not be an issue that the two antibodies are from the same host animal. This would only be a problem if you were going to detect the signal with an anti-mouse secondary antibody, because it would react with both the capture and detection antibodies. I hope this helps, please let me know if you need need any additional information.