Key features and details
- Biotin Rat monoclonal [5H10-27] to Integrin alpha 5
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: Biotin
- Isotype: IgG2a
Product nameBiotin Anti-Integrin alpha 5 antibody [5H10-27]
See all Integrin alpha 5 primary antibodies
DescriptionBiotin Rat monoclonal [5H10-27] to Integrin alpha 5
Specificityab25189 recognises alpha 5 subunit of the integrin alpha 5 beta 1 fibronectin receptor.
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
Tissue/ cell preparation of C57BL/6 x A/J F1 mast cell line MC/9 (Mouse)
The monoclonal antibody 5H10-27 reacts with the alpha 5 chain (VLA-5) of the CD49e/CD29 heterodimeric fibronectin receptor. Soluble 5H10-27 antibody inhibits VLA-5-mediated functions in vitro. Immobilized 5H10-27 has also been demonstrated to co-stimulate the proliferative response of CD8+ T cells to plate-bound anti-CD3 monoclonal antibody.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Preservative: 0.1% Sodium azide
Concentration information loading...
Primary antibody notesThe monoclonal antibody 5H10-27 reacts with the alpha 5 chain (VLA-5) of the CD49e/CD29 heterodimeric fibronectin receptor. Soluble 5H10-27 antibody inhibits VLA-5-mediated functions in vitro. Immobilized 5H10-27 has also been demonstrated to co-stimulate the proliferative response of CD8+ T cells to plate-bound anti-CD3 monoclonal antibody.
Light chain typekappa
Our Abpromise guarantee covers the use of ab25189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionIntegrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Sequence similaritiesBelongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
- Information by UniProt
- CD49 antigen-like family member E antibody
- CD49e antibody
- Fibronectin receptor subunit alpha antibody
ab25189 has not yet been referenced specifically in any publications.