Key features and details
- Biotin Rat monoclonal [439-9B] to Integrin beta 4
- Suitable for: Flow Cyt
- Reacts with: Cow, Human
- Conjugation: Biotin
- Isotype: IgG2b
Product nameBiotin Anti-Integrin beta 4 antibody [439-9B]
See all Integrin beta 4 primary antibodies
DescriptionBiotin Rat monoclonal [439-9B] to Integrin beta 4
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Cow, Human
CD104 (Integrin beta 4)
- EDTA treated A549 cell line
ab95584 has been reported to have no effect on adhesion of Human colon adenocarcinoma cells
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Primary antibody notesab95584 has been reported to have no effect on adhesion of Human colon adenocarcinoma cells
Our Abpromise guarantee covers the use of ab95584 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.25µg for 105-8 cells.
ab18542 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
FunctionIntegrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
Tissue specificityIntegrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
Involvement in diseaseDefects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
Sequence similaritiesBelongs to the integrin beta chain family.
Contains 1 Calx-beta domain.
Contains 4 fibronectin type-III domains.
Contains 1 PSI domain.
Contains 1 VWFA domain.
DomainThe fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
- Information by UniProt
- CD 104 antibody
- CD104 antibody
- CD104 antigen antibody
ab95584 has been referenced in 2 publications.
- Simon-Assmann P et al. Adhesive properties and integrin expression profiles of two colonic cancer populations differing by their spreading on laminin. J Cell Sci 107 ( Pt 3):577-87 (1994). PubMed: 8006072
- Falcioni R et al. Monoclonal antibody to human carcinoma-associated protein complex: quantitation in normal and tumor tissue. Cancer Res 48:816-21 (1988). PubMed: 2448027