Key features and details
- Biotin Rat monoclonal [FIB504] to Integrin beta 7
- Suitable for: ICC, Flow Cyt
- Reacts with: Mouse, Human
- Conjugation: Biotin
- Isotype: IgG2a
Product nameBiotin Anti-Integrin beta 7 antibody [FIB504]
See all Integrin beta 7 primary antibodies
DescriptionBiotin Rat monoclonal [FIB504] to Integrin beta 7
Tested applicationsSuitable for: ICC, Flow Cytmore details
Species reactivityReacts with: Mouse, Human
TK1 murine t-cell lymphoma
- Normal Human peripheral blood cells
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab95816 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|Flow Cyt||Use 0.125µg for 105 cells.
Range from 105 to 108 cells/test in a final volume of 100 µl.
ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionIntegrin alpha-4/beta-7 (Peyer patches-specific homing receptor LPAM-1) is an adhesion molecule that mediates lymphocyte migration and homing to gut-associated lymphoid tissue (GALT). Integrin alpha-4/beta-7 interacts with the cell surface adhesion molecules MADCAM1 which is normally expressed by the vascular endothelium of the gastrointestinal tract. Interacts also with VCAM1 and fibronectin, an extracellular matrix component. It recognizes one or more domains within the alternatively spliced CS-1 region of fibronectin. Interactions involves the tripeptide L-D-T in MADCAM1, and L-D-V in fibronectin. Binds to HIV-1 gp120, thereby allowing the virus to enter GALT, which is thought to be the major trigger of AIDS disease. Interaction would involve a tripeptide L-D-I in HIV-1 gp120. Integrin alpha-E/beta-7 (HML-1) is a receptor for E-cadherin.
Tissue specificityExpressed in a variety of leukocyte lines.
Sequence similaritiesBelongs to the integrin beta chain family.
Contains 1 VWFA domain.
DomainDomain I contains three cation-binding sites: the ligand-integrin-binding site (LIMBS), the metal ion-dependent adhesion site (MIDAS), and the adjacent to MIDAS site (ADMIDAS). In the absence of a ligand or in calcium-dependent binding, only ADMIDAS is occupied. In magnesium-dependent binding all three sites bind metal ions. LIMBS positively modify ligand binding whereas ADMIDAS negatively modify ligand binding.
- Information by UniProt
- Gut homing receptor beta subunit antibody
- Integrin beta 7 subunit antibody
- Integrin beta-7 antibody
ICC/IF image of Integrin beta 7 staining on RAW264.7 cell line using ab95816 (1:300). The cells were fixed using formaldehyde and permeabilized using 0.2% Triton X-100. The cells were blocked using 10% goat serum for 1 hour at 20°C. The cells were incubated with ab95816 (1:300) for 1 hour at 20°C. The staining was detected using Streptavedin conjugated to Alexo Fluor 647 (1:350)
Staining of normal Human peripheral blood cells with 0.06 µg of ab95816 followed by Streptavidin PE. Cells in the lymphocyte gate were used for analysis.
Staining of normal Human peripheral blood cells with Anti-Human CD3 APC and 0.125 µg of Rat IgG2a ? Isotype Control Biotin. Cells in the lymphocyte gate were used for analysis.
ab95816 has been referenced in 3 publications.
- Chai Z et al. Recombinant Viral Capsid Protein L2 (rVL2) of HPV 16 Suppresses Cell Proliferation and Glucose Metabolism via ITGB7/C/EBPß Signaling Pathway in Cervical Cancer Cell Lines. Onco Targets Ther 12:10415-10425 (2019). PubMed: 31819523
- Meerschaert J et al. Engagement of alpha4beta7 integrins by monoclonal antibodies or ligands enhances survival of human eosinophils in vitro. J Immunol 163:6217-27 (1999). PubMed: 10570314
- Tidswell M et al. Structure-function analysis of the integrin beta 7 subunit: identification of domains involved in adhesion to MAdCAM-1. J Immunol 159:1497-505 (1997). PubMed: 9233649