RecombinantRabMAb

Recombinant Biotin Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab201534)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab201534)
  • Biotin Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab201534)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Biotin Rabbit monoclonal [EP929Y] to Ionotropic glutamate receptor 2 + 3
  • Suitable for: IHC-P
  • Reacts with: Human
  • Conjugation: Biotin

Overview

  • Product name

    Biotin Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y]
    See all Ionotropic glutamate receptor 2 + 3 primary antibodies

  • Description

    Biotin Rabbit monoclonal [EP929Y] to Ionotropic glutamate receptor 2 + 3

  • Host species

    Rabbit

  • Conjugation

    Biotin

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human Ionotropic glutamate receptor 2 + 3. The exact sequence is proprietary.

  • Positive control

    • IHC-P: FFPE human hippocampus (normal) tissue sections.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab201534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    Ion channels activated by glutamate are typically divided into two classes. Those sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). AMPAR are comprised of four distinct Glutamate Receptor Subunits (GluR1-4) and they play key roles in almost all excitatory neurotransmission in the brain. The GluR2 subunit is widely expressed throughout the nervous system where it is thought to play key roles in synaptic plasticity and learning and memory.

  • Cellular localization

    Cell Membrane; multi-pass membrane protein.

  • Database links

    see all

  • Alternative names

    • AMPA selective glutamate receptor 2 antibody
    • AMPA selective glutamate receptor 3 antibody
    • GluR K2 antibody
    • GluR K3 antibody
    • GLURB antibody
    • GLURC antibody
    • GLUTAMATE RECEPTOR 2 antibody
    • GLUTAMATE RECEPTOR 3 antibody
    • Glutamate receptor ionotropic AMPA 2 antibody
    • Glutamate receptor ionotropic AMPA 3 antibody
    • GRIA2 antibody
    • GRIA3 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab201534)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab201534)

    IHC image of Ionotropic glutamate receptor 2 + 3 staining in a section of formalin-fixed paraffin-embedded normal human hippocampus tissue, performed on a Leica BondTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab201534, 1/500 dilution, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Biotin Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab201534)
    Biotin Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab201534)

References (0)

Publishing research using ab201534? Please let us know so that we can cite the reference in this datasheet.

ab201534 has not yet been referenced specifically in any publications.

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