Biotin Anti-IP10 antibody [6D4] (ab8099)

Reviews (2)Q&A (1)
Immunohistochemistry (Frozen sections) - Biotin Anti-IP10 antibody [6D4] (ab8099)
  • Flow Cytometry (Intracellular) - Biotin Anti-IP10 antibody [6D4] (ab8099)

Key features and details

  • Biotin Mouse monoclonal [6D4] to IP10
  • Suitable for: Flow Cyt (Intra), IHC-Fr
  • Reacts with: Human
  • Conjugation: Biotin
  • Isotype: IgG2a

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Carrier Free Unconjugated

Overview

  • Product name

    Biotin Anti-IP10 antibody [6D4]
    See all IP10 primary antibodies

  • Description

    Biotin Mouse monoclonal [6D4] to IP10

  • Host species

    Mouse

  • Conjugation

    Biotin

  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-Frmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to IP10.

  • Positive control

    • Flow Cyt (Intra): HUVEC cells; IHC-Fr: Human tonsil tissue.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab8099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab97679 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr
1/25.
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab97679 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr
1/25.

Target

  • Function

    Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3.

  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.

  • Post-translational
    modifications

    CXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • Interferon gamma induced factor MOB1, mouse, homolog of antibody
      • Interferon gamma induced protein 10 antibody
      • 10 kDa interferon gamma induced protein antibody
      • 10 kDa interferon gamma-induced protein antibody
      • C X C motif chemokine 10 antibody
      • C7 antibody
      • Chemokine (C X C motif) ligand 10 antibody
      • Chemokine CXC motif ligand 10 antibody
      • Crg 2 antibody
      • CRG2 antibody
      • CXCL10 antibody
      • CXCL10(1-73) antibody
      • CXL10_HUMAN antibody
      • Gamma IP10 antibody
      • Gamma-IP10 antibody
      • gIP 10 antibody
      • GIP10 antibody
      • IFI10 antibody
      • INP 10 antibody
      • INP10 antibody
      • Interferon activated gene 10 antibody
      • Interferon activated gene 10 antibody
      • Interferon gamma induced cell line antibody
      • Interferon inducible cytokine IP 10 antibody
      • Interferon inducible cytokine IP10 antibody
      • IP 10 antibody
      • IP-10 antibody
      • Mob 1 antibody
      • MOB1 antibody
      • Protein 10 from interferon (gamma) induced cell line antibody
      • SCYB10 antibody
      • Small inducible cytokine B10 antibody
      • Small inducible cytokine B10 precursor antibody
      • Small inducible cytokine subfamily B (Cys X Cys) member 10 antibody
      • Small inducible cytokine subfamily B CXC member 10 antibody
      • Small inducible cytokine subfamily B, member 10 antibody
      • Small-inducible cytokine B10 antibody
      see all

    Images

    • Immunohistochemistry (Frozen sections) - Biotin Anti-IP10 antibody [6D4] (ab8099)
      Immunohistochemistry (Frozen sections) - Biotin Anti-IP10 antibody [6D4] (ab8099)

      Immunohistochesmistry analysis of frozen sections of human tonsil tissue labeling IP10 with ab8099 at 1/25 dilution.

    • Flow Cytometry (Intracellular) - Biotin Anti-IP10 antibody [6D4] (ab8099)
      Flow Cytometry (Intracellular) - Biotin Anti-IP10 antibody [6D4] (ab8099)

      Detection of IP10 in HUVEC cells. Red, black and blue line represent the isotype control, cells only and ab8099 at 20 μg/ml, respectively.

    References (0)

    Publishing research using ab8099? Please let us know so that we can cite the reference in this datasheet.

    ab8099 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-IP10 antibody [6D4] (Biotin)

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Purified protein
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Loading amount
    0.2 µg
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 20 2018

    ELISA abreview for Anti-IP10 antibody [6D4] (Biotin)

     Excellent
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Human Serum (Blood)
    Specification
    Blood
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: 25°C
    Type
    Sandwich (Detection)
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 02 2018

    Question

    I see that you stock the following antibody:

    Anti-IP10 antibody [6D4] (ab8098)

    I would like to use this antibody to stain for mouse CXCL10 using flow cytometry.



    Your technical info states that this antibody cross reacts with mouse, and I was wondering:

    1.) What evidence you have to back this up?

    2.) Whether the same clone, but which is biotinylated, should also cross react with mouse CXCL10 (Anti-IP10 antibody [6D4] (Biotin) (a b8099))? I note that the technical sheet for the biotinylated version of the same clone does not list mouse cross reactivity.



    Kind regards,

    Read More
    Answer

    Thank you for contacting us.

    We got the information for mouse cross-reactivity from one of the references (PMID: 16721359) for this antibody, but haven't tested it ourselves. However, as both Flow Cytometry as well as mouse are listed as tested, you are 100% covered by our Abpromise guarantee.

    The biotinylated version of this antibody in our catalog is simply not as well described as the non-modified version, but as they are derived from the same clone, the modified antibody probably works in mouse as well..

    For your information, here is the note regarding theprotocol for intracellular staining: "activated plasmacytoid dendritic cells were fixed with 2% paraformaldehyde and permeabilized with 0.5% saponin. All steps were performed in presence of 0.5 M EDTA to avoid cell aggregation".


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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