Biotin Anti-Kappa light chain antibody [TB28-2] (ab239244)
![Flow Cytometry - Biotin Anti-Kappa light chain antibody [TB28-2] (ab239244)](https://www.abcam.com/ps/products/239/ab239244/Images/ab239244-343242-kappalightchainantibodytb282-flow-pbc-human.jpg "Flow Cytometry - Biotin Anti-Kappa light chain antibody [TB28-2] (ab239244)")
Key features and details
- Biotin Mouse monoclonal [TB28-2] to Kappa light chain
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Overview
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Product name
Biotin Anti-Kappa light chain antibody [TB28-2]
See all Kappa light chain primary antibodies -
Description
Biotin Mouse monoclonal [TB28-2] to Kappa light chain -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human Kappa light chain. Human IgG-kappa myeloma protein.
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Positive control
- Flow cyt: Human peripheral blood cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin. -
Clonality
Monoclonal -
Clone number
TB28-2 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab239244 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use a concentration of 2 µg/ml. |
Target
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Relevance
Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83. -
Cellular localization
Cytoplasmic and Secreted -
Database links
- Entrez Gene: 28901 Human
- Entrez Gene: 3514 Human
- Omim: 147200 Human
- SwissProt: P01601 Human
- SwissProt: P01834 Human
- Unigene: 449609 Human
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Alternative names
- HCAK 1 antibody
- HCAK1 antibody
- Ig kappa chain C region antibody
see all
Images
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Flow Cytometry - Biotin Anti-Kappa light chain antibody [TB28-2] (ab239244)
Surface staining of kappa light chains in human peripheral blood cells with ab239244 (TB28-2) biotin, streptavidin-PE.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab239244 has not yet been referenced specifically in any publications.
