Overview

  • Product name

    Anti-Biotin Ligase Epitope Tag antibody
  • Description

    Rabbit polyclonal to Biotin Ligase Epitope Tag
  • Host species

    Rabbit
  • Specificity

    ab106159 detects the minimal peptide substrate (GLNDIFEAQKIEWH) of biotin ligase (BirA): E. coli biotin ligase tag (BLT) and biotin acceptor peptide (BAP). The antibody will recognize these tags irrespective of whether the tag is biotinylated.
    Western blot analysis of Bacillus subtilis strains producing either BAP-tagged SPoIIIAH or BAP-tagged SPoIIQ showed strong and specific reactivity to the antisera.

  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Synthetic peptide corresponding to Escherichia coli Biotin Ligase Epitope Tag.
    Sequence:

    GLNDIFEAQKIEWH

  • Positive control

    • Whole cell lysates from Bacillus subtilis producing BAP-tagged SPoIIIAH or BAP-tagged SPoIIQ
  • General notes

    Dilute only prior to immediate use.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride, Whole serum
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000.

References

This product has been referenced in:

  • Johnson BS  et al. Biotin tagging of MeCP2 in mice reveals contextual insights into the Rett syndrome transcriptome. Nat Med 23:1203-1214 (2017). WB . Read more (PubMed: 28920956) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - nuclear (Hek293T)
Gel Running Conditions
Reduced Denaturing (4–15% Mini Protean TGX (Bio-Rad))
Loading amount
30 µg
Treatment
Biotin 50 uM for 24 h
Specification
Hek293T
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Oct 30 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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