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    biotin-ly75dec-205-antibody-nldc-145-ab51820.pdf

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Immunology Adaptive Immunity B Cells Non-CD
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Biotin Anti-LY75/DEC-205 antibody [NLDC-145] (ab51820)

  • Datasheet
  • SDS
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Key features and details

  • Biotin Rat monoclonal [NLDC-145] to LY75/DEC-205
  • Reacts with: Mouse
  • Conjugation: Biotin
  • Isotype: IgG2a

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Conjugation
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Overview

  • Product name

    Biotin Anti-LY75/DEC-205 antibody [NLDC-145]
    See all LY75/DEC-205 primary antibodies
  • Description

    Biotin Rat monoclonal [NLDC-145] to LY75/DEC-205
  • Host species

    Rat
  • Conjugation

    Biotin
  • Specificity

    Reacts with Mouse non lymphoid dendritic cells: interdigitating cells (IDC), veiled cells and Langerhans cells, thymic epithelial cells.
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to Mouse LY75/DEC-205.

  • Epitope

    The antigen is a protein of 205kDa molecular weight which is localized on the cell surface and intracellularly. The epitope has not been further characterized.
  • Positive control

    • The following tissues also tested positive: Lymph node, Peyer’s patch, Thymus and Skin.
  • General notes

    Previously labelled as LY75. 

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% KATHON™ CG/ICP
    Constituents: 0.5% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    NLDC-145
  • Isotype

    IgG2a
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • Non-CD
    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated

Associated products

  • Alternative Versions

    • FITC Anti-LY75/DEC-205 antibody [NLDC-145] (ab51817)
  • Isotype control

    • Biotin Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18445)

Applications

  • Application notes
    Flow Cyt: Use at an assay dependent dilution (PMID 19109143).
    IHC-Fr: Use at a concentration of 3 µg/ml.


    Is unsuitable for IHC-P.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment (By similarity). Causes reduced proliferation of B-lymphocytes.
    • Tissue specificity

      Expressed in spleen, thymus, colon and peripheral blood lymphocytes. Detected in myeloid and B lymphoid cell lines. Isoform 2 and isoform 3 are expressed in malignant Hodgkin lymphoma cells called Hodgkin and Reed-Sternberg (HRS) cells.
    • Sequence similarities

      Contains 10 C-type lectin domains.
      Contains 1 fibronectin type-II domain.
      Contains 1 ricin B-type lectin domain.
    • Post-translational
      modifications

      N-glycosylated.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession O60449 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 17076 Mouse
      • SwissProt: Q60767 Mouse
      • Unigene: 2074 Mouse
      • Alternative names

        • C-type lectin domain family 13 member B antibody
        • CD 205 antibody
        • CD205 antibody
        • CD205 antigen antibody
        • CLEC 13B antibody
        • CLEC13B antibody
        • DEC 205 antibody
        • DEC-205 antibody
        • DEC205 antibody
        • gp200 antibody
        • gp200 MR6 antibody
        • gp200-MR6 antibody
        • LY 75 antibody
        • Ly-75 antibody
        • LY75 antibody
        • LY75_HUMAN antibody
        • Lymphocyte antigen 75 antibody
        • Renal Cell Carcinoma (gp200) antibody
        see all

      Protocols

      • Flow cytometry protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (10)

      Publishing research using ab51820? Please let us know so that we can cite the reference in this datasheet.

      ab51820 has been referenced in 10 publications.

      • Wang JQ  et al. PARG regulates the proliferation and differentiation of DCs and T cells via PARP/NF-?B in tumour metastases of colon carcinoma. Oncol Rep 41:2657-2666 (2019). PubMed: 30864743
      • Reeh KA  et al. Ectopic TBX1 suppresses thymic epithelial cell differentiation and proliferation during thymus organogenesis. Development 141:2950-8 (2014). IHC-P ; Mouse . PubMed: 25053428
      • Jenkinson SR  et al. TRAF3 enforces the requirement for T cell cross-talk in thymic medullary epithelial development. Proc Natl Acad Sci U S A 110:21107-12 (2013). Mouse . PubMed: 24324158
      • Baik S  et al. Generation of both cortical and Aire(+) medullary thymic epithelial compartments from CD205(+) progenitors. Eur J Immunol 43:589-94 (2013). PubMed: 23299414
      • Sitnik KM  et al. Mesenchymal cells regulate retinoic acid receptor-dependent cortical thymic epithelial cell homeostasis. J Immunol 188:4801-9 (2012). Flow Cyt ; Mouse . PubMed: 22504647
      • Semmrich M  et al. Directed antigen targeting in vivo identifies a role for CD103+ dendritic cells in both tolerogenic and immunogenic T-cell responses. Mucosal Immunol 5:150-60 (2012). PubMed: 22166938
      • Shakib S  et al. Checkpoints in the development of thymic cortical epithelial cells. J Immunol 182:130-7 (2009). Flow Cyt ; Mouse . PubMed: 19109143
      • Swiggard WJ  et al. DEC-205, a 205-kDa protein abundant on mouse dendritic cells and thymic epithelium that is detected by the monoclonal antibody NLDC-145: purification, characterization, and N-terminal amino acid sequence. Cell Immunol 165:302-11 (1995). PubMed: 7553896
      • Breel M  et al. Dendritic cells of the mouse recognized by two monoclonal antibodies. Eur J Immunol 17:1555-9 (1987). PubMed: 3678361
      • Kraal G  et al. Langerhans' cells, veiled cells, and interdigitating cells in the mouse recognized by a monoclonal antibody. J Exp Med 163:981-97 (1986). PubMed: 3950549

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