Biotin Anti-LY75/DEC-205 antibody [NLDC-145] (ab51820)
Key features and details
- Biotin Rat monoclonal [NLDC-145] to LY75/DEC-205
- Reacts with: Mouse
- Conjugation: Biotin
- Isotype: IgG2a
Overview
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Product name
Biotin Anti-LY75/DEC-205 antibody [NLDC-145]
See all LY75/DEC-205 primary antibodies -
Description
Biotin Rat monoclonal [NLDC-145] to LY75/DEC-205 -
Host species
Rat -
Conjugation
Biotin -
Specificity
Reacts with Mouse non lymphoid dendritic cells: interdigitating cells (IDC), veiled cells and Langerhans cells, thymic epithelial cells. -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse LY75/DEC-205.
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Epitope
The antigen is a protein of 205kDa molecular weight which is localized on the cell surface and intracellularly. The epitope has not been further characterized. -
Positive control
- The following tissues also tested positive: Lymph node, Peyer’s patch, Thymus and Skin.
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General notes
Previously labelled as LY75.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.05% KATHON™ CG/ICP
Constituents: 0.5% BSA, 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
NLDC-145 -
Isotype
IgG2a -
Research areas
Associated products
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Isotype control
Applications
Application notes
Flow Cyt: Use at an assay dependent dilution (PMID 19109143).
IHC-Fr: Use at a concentration of 3 µg/ml.
Is unsuitable for IHC-P.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at a concentration of 3 µg/ml.
Is unsuitable for IHC-P.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment (By similarity). Causes reduced proliferation of B-lymphocytes. -
Tissue specificity
Expressed in spleen, thymus, colon and peripheral blood lymphocytes. Detected in myeloid and B lymphoid cell lines. Isoform 2 and isoform 3 are expressed in malignant Hodgkin lymphoma cells called Hodgkin and Reed-Sternberg (HRS) cells. -
Sequence similarities
Contains 10 C-type lectin domains.
Contains 1 fibronectin type-II domain.
Contains 1 ricin B-type lectin domain. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 17076 Mouse
- SwissProt: Q60767 Mouse
- Unigene: 2074 Mouse
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Alternative names
- C-type lectin domain family 13 member B antibody
- CD 205 antibody
- CD205 antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab51820 has been referenced in 10 publications.
- Wang JQ et al. PARG regulates the proliferation and differentiation of DCs and T cells via PARP/NF-?B in tumour metastases of colon carcinoma. Oncol Rep 41:2657-2666 (2019). PubMed: 30864743
- Reeh KA et al. Ectopic TBX1 suppresses thymic epithelial cell differentiation and proliferation during thymus organogenesis. Development 141:2950-8 (2014). IHC-P ; Mouse . PubMed: 25053428
- Jenkinson SR et al. TRAF3 enforces the requirement for T cell cross-talk in thymic medullary epithelial development. Proc Natl Acad Sci U S A 110:21107-12 (2013). Mouse . PubMed: 24324158
- Baik S et al. Generation of both cortical and Aire(+) medullary thymic epithelial compartments from CD205(+) progenitors. Eur J Immunol 43:589-94 (2013). PubMed: 23299414
- Sitnik KM et al. Mesenchymal cells regulate retinoic acid receptor-dependent cortical thymic epithelial cell homeostasis. J Immunol 188:4801-9 (2012). Flow Cyt ; Mouse . PubMed: 22504647
- Semmrich M et al. Directed antigen targeting in vivo identifies a role for CD103+ dendritic cells in both tolerogenic and immunogenic T-cell responses. Mucosal Immunol 5:150-60 (2012). PubMed: 22166938
- Shakib S et al. Checkpoints in the development of thymic cortical epithelial cells. J Immunol 182:130-7 (2009). Flow Cyt ; Mouse . PubMed: 19109143
- Swiggard WJ et al. DEC-205, a 205-kDa protein abundant on mouse dendritic cells and thymic epithelium that is detected by the monoclonal antibody NLDC-145: purification, characterization, and N-terminal amino acid sequence. Cell Immunol 165:302-11 (1995). PubMed: 7553896
- Breel M et al. Dendritic cells of the mouse recognized by two monoclonal antibodies. Eur J Immunol 17:1555-9 (1987). PubMed: 3678361
- Kraal G et al. Langerhans' cells, veiled cells, and interdigitating cells in the mouse recognized by a monoclonal antibody. J Exp Med 163:981-97 (1986). PubMed: 3950549