Key features and details
- Biotin Mouse monoclonal [E1] to M13
- Suitable for: WB, ELISA, Flow Cyt, IHC
- Reacts with: Mouse
- Conjugation: Biotin
- Isotype: IgG3
Product nameBiotin Anti-M13 antibody [E1]
See all M13 primary antibodies
DescriptionBiotin Mouse monoclonal [E1] to M13
SpecificityM13 antibody [E1] (ab17269) is specific for gp3 protein.
Tested applicationsSuitable for: WB, ELISA, Flow Cyt, IHCmore details
Species reactivityReacts with: Mouse
Full length M13 phage coat protein; Exact epitope not determined.
M13 antibody [E1] (ab17269) has numerous uses in screening phage display libraries, especially the screening of single chain antibody libraries.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.08% Sodium azide
Concentration information loading...
PurityProtein A purified
Purification notesM13 antibody [E1] (ab17269) is purified using 0.2µm sterile filter.
Primary antibody notesM13 antibody [E1] (ab17269) has numerous uses in screening phage display libraries, especially the screening of single chain antibody libraries.
Light chain typeunknown
Our Abpromise guarantee covers the use of ab17269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 90 kDa.|
|ELISA||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells.
ab97679 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IHC||Use at an assay dependent concentration.|
RelevanceThe bacteriophage known as "M13" forms the basis of cloning systems designed to easily introduce mutations into genes inserted into the phage genome. It also has been used in various "phage display" methodologies and combinatorial DNA and peptide libraries. Coat protein A (G3P) is necessary for adsorption of the virion onto the F-pilus of the host cell. There are about five copies of this protein per mature phage. Consists of three domains (N1, N2, and CT). The N2 domain interacts with the F pilus, whereas the N1 domain (connected to N2 by a flexible glycine-rich linker and tightly interacting with it on the phage) forms a complex with the C-terminal domain of tolA at later stages of the infection process. They are located at the adsorption end of the phage particle.
- Bacteriophage M13 antibody
- Coliphage M13 antibody
- Enterobacteria phage M13 antibody
ab17269 has not yet been referenced specifically in any publications.