Biotin Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody (ab83139)
Key features and details
- Biotin Rabbit polyclonal to Macrophage Inflammatory Protein 1 alpha / CCL3
- Suitable for: Sandwich ELISA, WB
- Reacts with: Mouse
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody
See all Macrophage Inflammatory Protein 1 alpha / CCL3 primary antibodies -
Description
Biotin Rabbit polyclonal to Macrophage Inflammatory Protein 1 alpha / CCL3 -
Host species
Rabbit -
Conjugation
Biotin -
Tested applications
Suitable for: Sandwich ELISA, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Highly pure (>98%) recombinant mouse Macrophage Inflammatory Protein 1 alpha/ CCL3
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Lyophilized:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab83139 is sterile filtered. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab83139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant murine Macrophage Inflammatory Protein 1 alpha / CCL3. |
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| WB | Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 10 kDa. The detection limit for recombinant murine Inflammatory Protein 1 alpha / CCL3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Target
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Function
Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsN-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 20302 Mouse
- SwissProt: P10855 Mouse
- Unigene: 1282 Mouse
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Alternative names
- C C motif chemokine 3 antibody
- CCL 3 antibody
- CCL3 antibody
see all
Images
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Western blot - Biotin Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody (ab83139)
To detect murine Macrophage Inflammatory Protein 1 alpha / CCL3 by Western Blot analysis ab83139 can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant murine Macrophage Inflammatory Protein 1 alpha / CCL3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Image shown is non-reducing conditions; Murine recombinant Macrophage Inflammatory Protein 1 alpha / CCL3 protein at 250 - 0.24 ng/ml.
Lane 1 is MW marker, lanes 2-12 murine recombinant Macrophage Inflammatory Protein 1 alpha / CCL3 protein.
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Sandwich ELISA - Biotin Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody (ab83139)To detect murine Macrophage Inflammatory Protein 1 alpha / CCL3 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab83139 is required. This biotinylated polyclonal antibody, in conjunction with an anti-Macrophage Inflammatory Protein 1 alpha / CCL3 (ab9927) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant murine Macrophage Inflammatory Protein 1 alpha / CCL3.
Datasheets and documents
References (0)
ab83139 has not yet been referenced specifically in any publications.
