Key features and details
- Biotin Rabbit polyclonal to Macrophage Inflammatory Protein 1 alpha / CCL3
- Suitable for: Sandwich ELISA, WB
- Reacts with: Mouse
- Conjugation: Biotin
- Isotype: IgG
Product nameBiotin Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody
See all Macrophage Inflammatory Protein 1 alpha / CCL3 primary antibodies
DescriptionBiotin Rabbit polyclonal to Macrophage Inflammatory Protein 1 alpha / CCL3
Tested applicationsSuitable for: Sandwich ELISA, WBmore details
Species reactivityReacts with: Mouse
Highly pure (>98%) recombinant mouse Macrophage Inflammatory Protein 1 alpha/ CCL3
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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FormLyophilized:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Purification notesab83139 is sterile filtered.
Our Abpromise guarantee covers the use of ab83139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml.
Allows the detection of at least 0.2 - 0.4 ng/well of recombinant murine Macrophage Inflammatory Protein 1 alpha / CCL3.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 10 kDa.
The detection limit for recombinant murine Inflammatory Protein 1 alpha / CCL3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
FunctionMonokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
modificationsN-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells.
- Information by UniProt
- C C motif chemokine 3 antibody
- CCL 3 antibody
- CCL3 antibody
To detect murine Macrophage Inflammatory Protein 1 alpha / CCL3 by Western Blot analysis ab83139 can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant murine Macrophage Inflammatory Protein 1 alpha / CCL3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Image shown is non-reducing conditions; Murine recombinant Macrophage Inflammatory Protein 1 alpha / CCL3 protein at 250 - 0.24 ng/ml.
Lane 1 is MW marker, lanes 2-12 murine recombinant Macrophage Inflammatory Protein 1 alpha / CCL3 protein.
To detect murine Macrophage Inflammatory Protein 1 alpha / CCL3 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab83139 is required. This biotinylated polyclonal antibody, in conjunction with an anti-Macrophage Inflammatory Protein 1 alpha / CCL3 (ab9927) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant murine Macrophage Inflammatory Protein 1 alpha / CCL3.
ab83139 has not yet been referenced specifically in any publications.