Biotin Anti-Macrophage inflammatory protein 5 antibody (ab83433)
Key features and details
- Biotin Goat polyclonal to Macrophage inflammatory protein 5
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-Macrophage inflammatory protein 5 antibody
See all Macrophage inflammatory protein 5 primary antibodies -
Description
Biotin Goat polyclonal to Macrophage inflammatory protein 5 -
Host species
Goat -
Conjugation
Biotin -
Tested applications
Suitable for: Sandwich ELISA, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Macrophage inflammatory protein 5 aa 22-113. Highly pure (>98%)
Sequence:QFTNDAETEL MMSKLPLENP VVLNSFHFAA DCCTSYISQS IPCSLMKSYF ETSSECSKPG VIFLTKKGRQ VCAKPSGPGV QDCMKKLKPY SI
Database link: Q16663 -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Lyophilized: Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Constituent: PBS -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
ab83433 is sterile filtered. This antibody was purified by affinity chromatography and then biotinylated. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab83433 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Macrophage inflammatory protein 5. |
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| WB | Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 12 kDa. The detection limit for recombinant human Macrophage inflammatory protein 5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Target
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Function
Chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are more potent chemoattractants than the small-inducible cytokine A15. -
Tissue specificity
Most abundant in heart, skeletal muscle and adrenal gland. Lower levels in placenta, liver, pancreas and bone marrow. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are found in high levels in synovial fluids from rheumatoid patients. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 6359 Human
- Omim: 601393 Human
- SwissProt: Q16663 Human
- Unigene: 272493 Human
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Alternative names
- C-C motif chemokine 15 antibody
- C-C motif chemokine ligand 15 antibody
- CC chemokine 3 antibody
see all
Images
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Western blot - Biotin Anti-Macrophage inflammatory protein 5 antibody (ab83433)
To detect human Macrophage inflammatory protein 5 by Western Blot analysis ab83433 can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human Macrophage inflammatory protein 5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Image shown is non-reducing conditions; human recombinant Macrophage inflammatory protein 5 protein at 250 - 0.24 ng/ml.
Lane 1 is MW marker, lanes 2-12 human recombinant Macrophage inflammatory protein 5 protein.
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Sandwich ELISA - Biotin Anti-Macrophage inflammatory protein 5 antibody (ab83433)To detect human Macrophage inflammatory protein 5 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab83433 is required. This biotinylated polyclonal antibody, in conjunction with an appropriate anti-Macrophage inflammatory protein 5 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human Macrophage inflammatory protein 5.
Datasheets and documents
References (0)
ab83433 has not yet been referenced specifically in any publications.
