Key features and details
- Biotin Goat polyclonal to Macrophage inflammatory protein 5
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Product nameBiotin Anti-Macrophage inflammatory protein 5 antibody
See all Macrophage inflammatory protein 5 primary antibodies
DescriptionBiotin Goat polyclonal to Macrophage inflammatory protein 5
Tested applicationsSuitable for: Sandwich ELISA, WBmore details
Species reactivityReacts with: Human
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FormLyophilized: Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Concentration information loading...
Purification notesab83433 is sterile filtered. This antibody was purified by affinity chromatography and then biotinylated.
Our Abpromise guarantee covers the use of ab83433 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml.
Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Macrophage inflammatory protein 5.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 12 kDa.
The detection limit for recombinant human Macrophage inflammatory protein 5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
FunctionChemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are more potent chemoattractants than the small-inducible cytokine A15.
Tissue specificityMost abundant in heart, skeletal muscle and adrenal gland. Lower levels in placenta, liver, pancreas and bone marrow. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are found in high levels in synovial fluids from rheumatoid patients.
Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
- Information by UniProt
- C-C motif chemokine 15 antibody
- C-C motif chemokine ligand 15 antibody
- CC chemokine 3 antibody
To detect human Macrophage inflammatory protein 5 by Western Blot analysis ab83433 can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human Macrophage inflammatory protein 5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Image shown is non-reducing conditions; human recombinant Macrophage inflammatory protein 5 protein at 250 - 0.24 ng/ml.
Lane 1 is MW marker, lanes 2-12 human recombinant Macrophage inflammatory protein 5 protein.
To detect human Macrophage inflammatory protein 5 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab83433 is required. This biotinylated polyclonal antibody, in conjunction with an appropriate anti-Macrophage inflammatory protein 5 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human Macrophage inflammatory protein 5.
ab83433 has not yet been referenced specifically in any publications.