Biotin Anti-MCP2 antibody (ab271251)
Key features and details
- Biotin Rabbit polyclonal to MCP2
- Suitable for: WB, ELISA
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-MCP2 antibody
See all MCP2 primary antibodies -
Description
Biotin Rabbit polyclonal to MCP2 -
Host species
Rabbit -
Conjugation
Biotin -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human MCP2 aa 1 to the C-terminus.
Database link: P80075 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 11 kDa.
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ELISA |
Use a concentration of 0.25 - 1 µg/ml.
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Notes |
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WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 11 kDa. |
ELISA
Use a concentration of 0.25 - 1 µg/ml. |
Target
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Function
Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8. -
Tissue specificity
Highest expression found in the small intestine and peripheral blood cells. Intermediate levels seen in the heart, placenta, lung, skeletal muscle, thymus, colon, ovary, spinal cord and pancreas. Low levels seen in the brain, liver, spleen and prostate. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsN-terminal processed form MCP-2(6-76) is produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Ccl8 antibody
- CCL8_HUMAN antibody
- HC14 antibody
see all
Images
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Western blot analysis of varying levels of Recombinant human MCP-2 protein using ab271251 at 0.1 µg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant MCP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Human MCP2 was detected by sandwich ELISA (using 100 µl/well) using a concentration of 0.25-1.0 µg/ml of ab271251. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant MCP2 protein.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab271251 has not yet been referenced specifically in any publications.