Biotin Anti-Menin antibody (ab265593)
Key features and details
- Biotin Goat polyclonal to Menin
- Suitable for: WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-Menin antibody
See all Menin primary antibodies -
Description
Biotin Goat polyclonal to Menin -
Host species
Goat -
Conjugation
Biotin -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide within Human Menin aa 575-615. The exact sequence is proprietary. NP_000235.2
Database link: O00255 -
Positive control
- WB: HEK-293T whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Lyophilized:For 0.1 mg reconstitute with 100 µl di-water. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 20% Trehalose, 0.2% BSA
Lyophilized from. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab265593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 68 kDa. |
Target
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Function
Essential component of a MLL/SET1 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3 (H3K4). Functions as a transcriptional regulator. Binds to the TERT promoter and represses telomerase expression. Plays a role in TGFB1-mediated inhibition of cell-proliferation, possibly regulating SMAD3 transcriptional activity. Represses JUND-mediated transcriptional activation on AP1 sites, as well as that mediated by NFKB subunit RELA. Positively regulates HOXC8 and HOXC6 gene expression. May be involved in normal hematopoiesis through the activation of HOXA9 expression (By similarity). May be involved in DNA repair. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Defects in MEN1 are the cause of familial multiple endocrine neoplasia type I (MEN1) [MIM:131100]. Autosomal dominant disorder characterized by tumors of the parathyroid glands, gastro-intestinal endocrine tissue, the anterior pituitary and other tissues. Cutaneous lesions and nervous-tissue tumors can exist. Prognosis in MEN1 patients is related to hormonal hypersecretion by tumors, such as hypergastrinemia causing severe peptic ulcer disease (Zollinger-Ellison syndrome, ZES), primary hyperparathyroidism, and acute forms of hyperinsulinemia.
Defects in MEN1 are the cause of familial isolated hyperparathyroidism (FIHP) [MIM:145000]; also known as hyperparathyroidism type 1 (HRPT1). FIHP is an autosomal dominant disorder characterized by hypercalcemia, elevated parathyroid hormone (PTH) levels, and uniglandular or multiglandular parathyroid tumors. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Concentrated in nuclear body-like structures. Relocates to the nuclear matrix upon gamma irradiation. - Information by UniProt
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Database links
- Entrez Gene: 539431 Cow
- Entrez Gene: 483758 Dog
- Entrez Gene: 100722977 Guinea pig
- Entrez Gene: 100050500 Horse
- Entrez Gene: 4221 Human
- Entrez Gene: 17283 Mouse
- Entrez Gene: 100523533 Pig
- Entrez Gene: 29417 Rat
see all -
Alternative names
- MEA 1 antibody
- MEA1 antibody
- MEN 1 antibody
see all
Images
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Western blot - Biotin Anti-Menin antibody (ab265593)All lanes : Biotin Anti-Menin antibody (ab265593) at 1 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2 : HEK-293T whole cell lysate at 15 µg
Lane 3 : HEK-293T whole cell lysate at 5 µg
Predicted band size: 68 kDa
Exposure time: 30 secondsDetection: Streptavidin-HRP and chemiluminescence.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab265593 has not yet been referenced specifically in any publications.
