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Immunology Innate Immunity Macrophage / Inflamm.
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Biotin Anti-Metallophilic Macrophages antibody [MOMA-1] (ab51814)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (14)

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Immunohistochemistry (Frozen sections) - Biotin Anti-Metallophilic Macrophages antibody [MOMA-1] (ab51814)

    Key features and details

    • Biotin Rat monoclonal [MOMA-1] to Metallophilic Macrophages
    • Suitable for: IHC-Fr
    • Reacts with: Mouse
    • Conjugation: Biotin
    • Isotype: IgG2a

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    Overview

    • Product name

      Biotin Anti-Metallophilic Macrophages antibody [MOMA-1]
      See all Metallophilic Macrophages primary antibodies
    • Description

      Biotin Rat monoclonal [MOMA-1] to Metallophilic Macrophages
    • Host species

      Rat
    • Conjugation

      Biotin
    • Specificity

      Does not react with dendritic cells, peritoneal resident macrophages, peritoneal exudate cells, bone marrow or blood cells.
    • Tested applications

      Suitable for: IHC-Frmore details
      Unsuitable for: IHC-P
    • Species reactivity

      Reacts with: Mouse
    • Immunogen

      Mouse lymph node tissue

    • Positive control

      • Mouse spleen
    • General notes

      The antigen is differentially induced in in vitro derived macrophages depending on the colony-stimulating factor applied (IL3 > M-CSF > GM-CSF). Distinct macrophage subpopulations of lymphoid organs express the antigen that reacts with this antibody. In the spleen, they are localized at the marginal sinus forming a ring around the periarteriolar lymphocyte sheath and follicular areas at the inner side of marginal zones. In lymph nodes, they are localized in the sinusoids and medullary cords, but not within follicular areas or paracortex. In Peyer’s patches they are localized in the interfollicular areas at the serosal side. Kupffer cells in the liver can be clearly stained. No reactive macrophages were found in the thymus, brain, kidney, liver, skin or heart. In non-lymphoid organs, the antigen is only found on a macrophage subpopulation in the lamina propria of the villi of the small intestine.


      Monoclonal antibody MOMA-1 is a useful marker for the identification of macrophage subpopulations in various organs, mostly characterized by a high level of non-specific esterase expression. Staining is particularly noteworthy with the metallophilic macrophages adjacent to the marginal zone of the spleen. MOMA-1 is also very suitable for differentiation of non-metallophilic marginal zone macrophages as detected by the monoclonal antibody ER-TR9 (ab51819). In addition, MOMA-1 detects macrophages at inflammatory sites and is positive with Kupffer cells.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.20
      Preservative: 0.05% KATHON™ CG/ICP
      Constituents: 0.5% BSA, 99% PBS
    • Concentration information loading...
    • Purity

      Affinity purified
    • Primary antibody notes

      Monoclonal antibody MOMA-1 is a useful marker for the identification of macrophage subpopulations in various organs, mostly characterized by a high level of non-specific esterase expression. Staining is particularly noteworthy with the metallophilic macrophages adjacent to the marginal zone of the spleen. MOMA-1 is also very suitable for differentiation of non-metallophilic marginal zone macrophages as detected by the monoclonal antibody ER-TR9 (ab51819). In addition, MOMA-1 detects macrophages at inflammatory sites and is positive with Kupffer cells.
    • Clonality

      Monoclonal
    • Clone number

      MOMA-1
    • Isotype

      IgG2a
    • Research areas

      • Immunology
      • Innate Immunity
      • Macrophage / Inflamm.

    Associated products

    • Alternative Versions

      • Anti-Metallophilic Macrophages antibody [MOMA-1] (ab183549)
    • Isotype control

      • Biotin Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18445)
    • Related Products

      • Biotin Anti-CD209 antibody [ER-TR9] (ab51819)

    Applications

    Our Abpromise guarantee covers the use of ab51814 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-Fr Use a concentration of 0.5 - 1 µg/ml.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Relevance

      Metallophilic macrophages are a subpopulation of mature resident tissue macrophages. They show high non specific esterase activity and can be distinguished from splenic marginal zone macrophages by antibody staining and the lack of FITC-Ficoll uptake.

    Images

    • Immunohistochemistry (Frozen sections) - Biotin Anti-Metallophilic Macrophages antibody [MOMA-1] (ab51814)
      Immunohistochemistry (Frozen sections) - Biotin Anti-Metallophilic Macrophages antibody [MOMA-1] (ab51814)

      ab51814 at 0.5 µg/ml staining mouse spleen; frozen tissue.

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (14)

    Publishing research using ab51814? Please let us know so that we can cite the reference in this datasheet.

    ab51814 has been referenced in 14 publications.

    • Frenkel D  et al. Trypanosoma brucei Co-opts NK Cells to Kill Splenic B2 B Cells. PLoS Pathog 12:e1005733 (2016). PubMed: 27403737
    • Cuenca M  et al. Targeting of Ly9 (CD229) Disrupts Marginal Zone and B1 B Cell Homeostasis and Antibody Responses. J Immunol 196:726-37 (2016). PubMed: 26667173
    • Schaefer-Klein JL  et al. Topoisomerase 2 Alpha Cooperates with Androgen Receptor to Contribute to Prostate Cancer Progression. PLoS One 10:e0142327 (2015). WB . PubMed: 26560244
    • Yokogawa M  et al. Epicutaneous application of toll-like receptor 7 agonists leads to systemic autoimmunity in wild-type mice: a new model of systemic Lupus erythematosus. Arthritis Rheumatol 66:694-706 (2014). PubMed: 24574230
    • Khan TN  et al. Prolonged apoptotic cell accumulation in germinal centers of Mer-deficient mice causes elevated B cell and CD4+ Th cell responses leading to autoantibody production. J Immunol 190:1433-46 (2013). Mouse . PubMed: 23319738
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Hi,

    I am interested on find an Ab anti-CD169-biotin (anti-mouse) which is referenced as “anti–CD169-biotin [clone MOMA-1; AbCam]” in the paper Chow Aet al. Bone marrow CD169+ macrophages promote the retention of hematopoietic stem and progenitor cells in the mesenchymal stem cell niche. J Exp Med 208:261-71 (2011). I have checked Abcam catalogue and I have found “Anti-Metallophilic Macrophages antibody [MOMA-1] (Biotin) (ab51814)”. I would like to know if this Ab is the same one which is used in the paper I have mentioned.
    Thanks in advance,
    Best,

    Read More

    Abcam community

    Verified customer

    Asked on Feb 06 2012

    Answer

    Espero que no te importe que te conteste en castellano, ya que recientemente hemos inaugurado el Servicio Técnico en español. Si prefirieses seguir contactándonos en ingles, no dudes en comunicármelo.

    El artículo que mencionas se refiere exactamente al anticuerpo ab51814. Como puedes ver en su datasheet, en la pestaña “Specific References”, es el primero que se nombra.

    Espero haberte ayudado. Si tienes cualquier otra consulta, por favor, no dudes en contactarnos de nuevo.

    Read More

    Abcam Scientific Support

    Answered on Feb 06 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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