Biotin Anti-Metallophilic Macrophages antibody [MOMA-1] (ab51814)
![Immunohistochemistry (Frozen sections) - Biotin Anti-Metallophilic Macrophages antibody [MOMA-1] (ab51814)](https://www.abcam.com/ps/products/51/ab51814/Images/ab51814-63853-anti-metallophilic-macrophages-antibody-moma-1-biotin-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - Biotin Anti-Metallophilic Macrophages antibody [MOMA-1] (ab51814)")
Key features and details
- Biotin Rat monoclonal [MOMA-1] to Metallophilic Macrophages
- Suitable for: IHC-Fr
- Reacts with: Mouse
- Conjugation: Biotin
- Isotype: IgG2a
Overview
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Product name
Biotin Anti-Metallophilic Macrophages antibody [MOMA-1] -
Description
Biotin Rat monoclonal [MOMA-1] to Metallophilic Macrophages -
Host species
Rat -
Conjugation
Biotin -
Specificity
Does not react with dendritic cells, peritoneal resident macrophages, peritoneal exudate cells, bone marrow or blood cells. -
Tested applications
Suitable for: IHC-Frmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse Metallophilic Macrophages.
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Positive control
- Mouse spleen
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General notes
The antigen is differentially induced in in vitro derived macrophages depending on the colony-stimulating factor applied (IL3 > M-CSF > GM-CSF). Distinct macrophage subpopulations of lymphoid organs express the antigen that reacts with this antibody. In the spleen, they are localized at the marginal sinus forming a ring around the periarteriolar lymphocyte sheath and follicular areas at the inner side of marginal zones. In lymph nodes, they are localized in the sinusoids and medullary cords, but not within follicular areas or paracortex. In Peyer’s patches they are localized in the interfollicular areas at the serosal side. Kupffer cells in the liver can be clearly stained. No reactive macrophages were found in the thymus, brain, kidney, liver, skin or heart. In non-lymphoid organs, the antigen is only found on a macrophage subpopulation in the lamina propria of the villi of the small intestine.Monoclonal antibody MOMA-1 is a useful marker for the identification of macrophage subpopulations in various organs, mostly characterized by a high level of non-specific esterase expression. Staining is particularly noteworthy with the metallophilic macrophages adjacent to the marginal zone of the spleen. MOMA-1 is also very suitable for differentiation of non-metallophilic marginal zone macrophages as detected by the monoclonal antibody ER-TR9 (ab51819). In addition, MOMA-1 detects macrophages at inflammatory sites and is positive with Kupffer cells.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.05% KATHON™ CG/ICP
Constituents: 0.5% BSA, 99% PBS -
Concentration information loading... -
Purity
Affinity purified -
Primary antibody notes
Monoclonal antibody MOMA-1 is a useful marker for the identification of macrophage subpopulations in various organs, mostly characterized by a high level of non-specific esterase expression. Staining is particularly noteworthy with the metallophilic macrophages adjacent to the marginal zone of the spleen. MOMA-1 is also very suitable for differentiation of non-metallophilic marginal zone macrophages as detected by the monoclonal antibody ER-TR9 (ab51819). In addition, MOMA-1 detects macrophages at inflammatory sites and is positive with Kupffer cells. -
Clonality
Monoclonal -
Clone number
MOMA-1 -
Isotype
IgG2a -
Research areas
Associated products
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab51814 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use a concentration of 0.5 - 1 µg/ml.
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| Notes |
|---|
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IHC-Fr
Use a concentration of 0.5 - 1 µg/ml. |
Target
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Relevance
Metallophilic macrophages are a subpopulation of mature resident tissue macrophages. They show high non specific esterase activity and can be distinguished from splenic marginal zone macrophages by antibody staining and the lack of FITC-Ficoll uptake.
Images
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Immunohistochemistry (Frozen sections) - Biotin Anti-Metallophilic Macrophages antibody [MOMA-1] (ab51814)
ab51814 at 0.5 µg/ml staining mouse spleen; frozen tissue.
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab51814 has been referenced in 16 publications.
- Kobia FM et al. Notch dimerization and gene dosage are important for normal heart development, intestinal stem cell maintenance, and splenic marginal zone B-cell homeostasis during mite infestation. PLoS Biol 18:e3000850 (2020). PubMed: 33017398
- Ádori M et al. Altered Marginal Zone B Cell Selection in the Absence of I?BNS. J Immunol 200:775-787 (2018). PubMed: 29222168
- Frenkel D et al. Trypanosoma brucei Co-opts NK Cells to Kill Splenic B2 B Cells. PLoS Pathog 12:e1005733 (2016). PubMed: 27403737
- Cuenca M et al. Targeting of Ly9 (CD229) Disrupts Marginal Zone and B1 B Cell Homeostasis and Antibody Responses. J Immunol 196:726-37 (2016). PubMed: 26667173
- Schaefer-Klein JL et al. Topoisomerase 2 Alpha Cooperates with Androgen Receptor to Contribute to Prostate Cancer Progression. PLoS One 10:e0142327 (2015). WB . PubMed: 26560244