Key features and details
- Biotin Rat monoclonal [MOMA-1] to Metallophilic Macrophages
- Suitable for: IHC-Fr
- Reacts with: Mouse
- Conjugation: Biotin
- Isotype: IgG2a
Product nameBiotin Anti-Metallophilic Macrophages antibody [MOMA-1]
See all Metallophilic Macrophages primary antibodies
DescriptionBiotin Rat monoclonal [MOMA-1] to Metallophilic Macrophages
SpecificityDoes not react with dendritic cells, peritoneal resident macrophages, peritoneal exudate cells, bone marrow or blood cells.
Tested applicationsSuitable for: IHC-Frmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse
Mouse lymph node tissue
- Mouse spleen
General notesThe antigen is differentially induced in in vitro derived macrophages depending on the colony-stimulating factor applied (IL3 > M-CSF > GM-CSF). Distinct macrophage subpopulations of lymphoid organs express the antigen that reacts with this antibody. In the spleen, they are localized at the marginal sinus forming a ring around the periarteriolar lymphocyte sheath and follicular areas at the inner side of marginal zones. In lymph nodes, they are localized in the sinusoids and medullary cords, but not within follicular areas or paracortex. In Peyer’s patches they are localized in the interfollicular areas at the serosal side. Kupffer cells in the liver can be clearly stained. No reactive macrophages were found in the thymus, brain, kidney, liver, skin or heart. In non-lymphoid organs, the antigen is only found on a macrophage subpopulation in the lamina propria of the villi of the small intestine.
Monoclonal antibody MOMA-1 is a useful marker for the identification of macrophage subpopulations in various organs, mostly characterized by a high level of non-specific esterase expression. Staining is particularly noteworthy with the metallophilic macrophages adjacent to the marginal zone of the spleen. MOMA-1 is also very suitable for differentiation of non-metallophilic marginal zone macrophages as detected by the monoclonal antibody ER-TR9 (ab51819). In addition, MOMA-1 detects macrophages at inflammatory sites and is positive with Kupffer cells.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.05% KATHON™ CG/ICP
Constituents: 0.5% BSA, 99% PBS
Concentration information loading...
Primary antibody notesMonoclonal antibody MOMA-1 is a useful marker for the identification of macrophage subpopulations in various organs, mostly characterized by a high level of non-specific esterase expression. Staining is particularly noteworthy with the metallophilic macrophages adjacent to the marginal zone of the spleen. MOMA-1 is also very suitable for differentiation of non-metallophilic marginal zone macrophages as detected by the monoclonal antibody ER-TR9 (ab51819). In addition, MOMA-1 detects macrophages at inflammatory sites and is positive with Kupffer cells.
Our Abpromise guarantee covers the use of ab51814 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use a concentration of 0.5 - 1 µg/ml.|
RelevanceMetallophilic macrophages are a subpopulation of mature resident tissue macrophages. They show high non specific esterase activity and can be distinguished from splenic marginal zone macrophages by antibody staining and the lack of FITC-Ficoll uptake.
ab51814 has been referenced in 14 publications.
- Frenkel D et al. Trypanosoma brucei Co-opts NK Cells to Kill Splenic B2 B Cells. PLoS Pathog 12:e1005733 (2016). PubMed: 27403737
- Cuenca M et al. Targeting of Ly9 (CD229) Disrupts Marginal Zone and B1 B Cell Homeostasis and Antibody Responses. J Immunol 196:726-37 (2016). PubMed: 26667173
- Schaefer-Klein JL et al. Topoisomerase 2 Alpha Cooperates with Androgen Receptor to Contribute to Prostate Cancer Progression. PLoS One 10:e0142327 (2015). WB . PubMed: 26560244
- Yokogawa M et al. Epicutaneous application of toll-like receptor 7 agonists leads to systemic autoimmunity in wild-type mice: a new model of systemic Lupus erythematosus. Arthritis Rheumatol 66:694-706 (2014). PubMed: 24574230
- Khan TN et al. Prolonged apoptotic cell accumulation in germinal centers of Mer-deficient mice causes elevated B cell and CD4+ Th cell responses leading to autoantibody production. J Immunol 190:1433-46 (2013). Mouse . PubMed: 23319738