Biotin Anti-MHC class II I E kappa antibody [14-4-4S] (ab93504)
Key features and details
- Biotin Mouse monoclonal [14-4-4S] to MHC class II I E kappa
- Reacts with: Mouse, Rat
- Conjugation: Biotin
- Isotype: IgG2a
Overview
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Product name
Biotin Anti-MHC class II I E kappa antibody [14-4-4S]
See all MHC class II I E kappa primary antibodies -
Description
Biotin Mouse monoclonal [14-4-4S] to MHC class II I E kappa -
Host species
Mouse -
Conjugation
Biotin -
Specificity
ab93504 reacts with the mouse and rat MHC Class II, I-E subregion-encoded glycoprotein in mice strains of H-2k, H-2d, H-2p and H-2r. H-2f and H-2s haplotypes do not express the I-E antigen. -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Tissue/ cell preparation: C3H mouse skin graft and splenocytes
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Positive control
- Mouse splenocytes
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General notes
The 14-4-4S monoclonal antibody has been reported to block antigen presentation in in vitro assays.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
The 14-4-4S monoclonal antibody has been reported to block antigen presentation in in vitro assays. -
Clonality
Monoclonal -
Clone number
14-4-4S -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
Target
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Relevance
A major histocompatibility complex class II receptor. These display processed antigens from virally infected or transformed cells. Class II positive cells ('antigen presenting cells') can take up antigens from outside by endocytosis, degrade them into small peptides, and re export the peptides (now bound to MHC class II protein) to the cell surface. These peptide MHC class II complexes can then be recognized by specific CD4+ lymphocytes. -
Cellular localization
Type I membrane protein -
Alternative names
- H2 Ea antibody
- H2-Ea antibody
Datasheets and documents
References (4)
ab93504 has been referenced in 4 publications.
- Spencer JS et al. Expression and function of mixed isotype MHC class II molecules in normal mice. J Immunol 151:6822-32 (1993). PubMed: 8258692
- Pruitt SK et al. The effect of xenoreactive antibody and B cell depletion on hyperacute rejection of guinea pig-to-rat cardiac xenografts. Transplantation 56:1318-24 (1993). PubMed: 8278996
- Liu H et al. Immune T cells sorted by flow cytometry confer protection against infection with Treponema pallidum subsp. pertenue in hamsters. Infect Immun 58:1685-90 (1990). PubMed: 2187804
- Maloy WL et al. Evidence for the association of I-A and I-E molecules in d-haplotype mice. Mol Immunol 23:263-9 (1986). PubMed: 3459016
