Biotin Mouse IgG2a, kappa [MOPC-173] - Isotype Control (ab18454)

Q&A (1)

Overview

  • Product name

    Biotin Mouse IgG2a, kappa [MOPC-173] - Isotype Control
    See all Mouse isotype controls

  • Conjugation

    Biotin

  • Specificity

    ab18454 MOPC-173 immunoglobulin has unknown specificity. ab18454 MOPC-173 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed rat and human tissues.

  • Tested applications

    Suitable for: Flow Cytmore details

  • General notes

    ab18454 MOPC-173 immunoglobulin is useful as an isotype-matched control for Western blotting, immunoprecipitation, immunohistochemical staining, and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    ab18454 antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.

  • Isotype control notes

    ab18454 MOPC-173 immunoglobulin is useful as an isotype-matched control for Western blotting, immunoprecipitation, immunohistochemical staining, and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

  • Clonality

    Monoclonal

  • Clone number

    MOPC-173

  • Isotype

    IgG2a

  • Light chain type

    kappa

  • Research areas

  • Alternative names

    • Mouse Isotype Control

Applications

Our Abpromise guarantee covers the use of ab18454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Suitable for surface or intracellular staining.

References (0)

Publishing research using ab18454? Please let us know so that we can cite the reference in this datasheet.

ab18454 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Q&A
Submit a question

Question

Dear Abcam,
I would like to ask a question about which control IgG I need to use for
28-14-8 (biotin) (#ab25237)
I want to use same isotype with biotin conjugated antibody and I found that ab 128976, which is biotinylated
Goat anti-Mouse IgG, but I am not sure if this is the right one to use.
Your kind help would be much appreciated.
Thanks a lot.

Read More
Answer

Thank you for contacting us.

I wouldn't recommend using ab128976 as an isotype control with ab25237 since ab128976 is raised in a goat and it needs to match the primary antibody you're using.

I would recommend ab18454 as an isotype control since this matches the species, isotype, clonality and conjugation of the primary antibody you're using.

https://www.abcam.com/Mouse-IgG2a-kappa-monoclonal-MOPC-173-biotin-Isotype-control-ab18454.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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