Biotin Mouse IgG2a, kappa [MOPC-173] - Isotype Control (ab18454)

Q&A (1)

Overview

  • Product name

    Biotin Mouse IgG2a, kappa [MOPC-173] - Isotype Control
    See all Mouse isotype controls

  • Conjugation

    Biotin

  • Specificity

    ab18454 MOPC-173 immunoglobulin has unknown specificity. ab18454 MOPC-173 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed rat and human tissues.

  • Tested applications

    Suitable for: Flow Cytmore details

  • General notes

    ab18454 MOPC-173 immunoglobulin is useful as an isotype-matched control for Western blotting, immunoprecipitation, immunohistochemical staining, and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    ab18454 antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.

  • Isotype control notes

    ab18454 MOPC-173 immunoglobulin is useful as an isotype-matched control for Western blotting, immunoprecipitation, immunohistochemical staining, and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

  • Clonality

    Monoclonal

  • Clone number

    MOPC-173

  • Isotype

    IgG2a

  • Light chain type

    kappa

  • Research areas

  • Alternative names

    • Mouse Isotype Control

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab18454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration.

Suitable for surface or intracellular staining.
Notes
Flow Cyt
Use at an assay dependent concentration.

Suitable for surface or intracellular staining.

References (0)

Publishing research using ab18454? Please let us know so that we can cite the reference in this datasheet.

ab18454 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Q&A
Submit a question

Question

Dear Abcam,
I would like to ask a question about which control IgG I need to use for
28-14-8 (biotin) (#ab25237)
I want to use same isotype with biotin conjugated antibody and I found that ab 128976, which is biotinylated
Goat anti-Mouse IgG, but I am not sure if this is the right one to use.
Your kind help would be much appreciated.
Thanks a lot.

Read More
Answer

Thank you for contacting us.

I wouldn't recommend using ab128976 as an isotype control with ab25237 since ab128976 is raised in a goat and it needs to match the primary antibody you're using.

I would recommend ab18454 as an isotype control since this matches the species, isotype, clonality and conjugation of the primary antibody you're using.

https://www.abcam.com/Mouse-IgG2a-kappa-monoclonal-MOPC-173-biotin-Isotype-control-ab18454.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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