Key features and details
- Biotin Chicken polyclonal to MRP8 + MRP14
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgY
Product nameBiotin Anti-MRP8 + MRP14 antibody
See all MRP8 + MRP14 primary antibodies
DescriptionBiotin Chicken polyclonal to MRP8 + MRP14
SpecificityDoes not discriminate between the individual subunits MRP8 or MRP14.
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Recombinant MRP8/14 heterocomplex (Human). The antigen is produced by the Ca2+ dependent heterocomplex formation of MRP8 (S100A8 or Calgranulin A) and MRP14 (S100A9 or Calgranulin B), two calcium binding proteins of the S 100 protein family.
- Human tonsil.
General notesPeripheral blood monocytes carry the antigen extra and intracellularly, neutrophils only intracellularly. The antigen is present on macrophages in the red pulp of the spleen and in the liver. It is also strongly expressed on macrophages in acute inflamed tissues (peridontitis, contact excema, urticaria, erythrodermia).
In immune histochemistry, this antibody detects early inflammatory macrophages and can thus be used for the classification of acute stage inflammation in tissue sections and in smears.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Concentration information loading...
Purification notesPurified from egg yolk by repeated precipitation.
Primary antibody notesIn immune histochemistry, this antibody detects early inflammatory macrophages and can thus be used for the classification of acute stage inflammation in tissue sections and in smears.
Our Abpromise guarantee covers the use of ab51863 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 4 µg/ml.
1/250. Proteinase K pretreatment for antigen retrieval is recommended.
RelevanceMRP8 and MRP14 are members of the S100 family of proteins containing 2 EF hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21.
Cellular localizationCytoplasmic and Nuclear
- CAGA antibody
- CAGB antibody
- Calgranulin A antibody
ab51863 has been referenced in 1 publication.
- Lood C et al. Protein synthesis of the pro-inflammatory S100A8/A9 complex in plasmacytoid dendritic cells and cell surface S100A8/A9 on leukocyte subpopulations in systemic lupus erythematosus. Arthritis Res Ther 13:R60 (2011). ELISA . PubMed: 21492422