Biotin Anti-Myc tag antibody [9E10] - C-terminal (ab197139)

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Key features and details

  • Biotin Mouse monoclonal [9E10] to Myc tag - C-terminal
  • Suitable for: Flow Cyt
  • Conjugation: Biotin
  • Isotype: IgG1

Overview

  • Product name

    Biotin Anti-Myc tag antibody [9E10] - C-terminal
    See all Myc tag primary antibodies

  • Description

    Biotin Mouse monoclonal [9E10] to Myc tag - C-terminal

  • Host species

    Mouse

  • Conjugation

    Biotin

  • Specificity

    This antibody is specific for Myc tagged proteins. The Myc tag epitope (EQKLISEEDL) is located at the dimerization site of c-myc and therefore this antibody does not perform well at recognizing endogenous c-myc.

  • Tested applications

    Suitable for: Flow Cytmore details

  • Immunogen

    Synthetic peptide corresponding to Human Myc tag aa 408-420 (C terminal).
    Sequence:

    AEEQKLISEEDLL


    Database link: P01106
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab197139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance

    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

  • Cellular localization

    Nuclear

  • Alternative names

    • c-myc tag antibody
    • Myc Epitope Tag antibody

References (0)

Publishing research using ab197139? Please let us know so that we can cite the reference in this datasheet.

ab197139 has not yet been referenced specifically in any publications.

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