Biotin Anti-Myc tag antibody (ab34773)
Key features and details
- Biotin Rabbit polyclonal to Myc tag
- Suitable for: ELISA, WB
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-Myc tag antibody
See all Myc tag primary antibodies -
Description
Biotin Rabbit polyclonal to Myc tag -
Host species
Rabbit -
Conjugation
Biotin -
Specificity
This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxytermini of targeted proteins in transfected or transformed cells. -
Tested applications
Suitable for: ELISA, WBmore details -
Immunogen
Synthetic peptide corresponding to Myc tag aa 410-419 conjugated to Keyhole Limpet Haemocyanin (KLH).
Sequence:EQKLISEEDL
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General notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab34773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/10000. | |
| WB | Use a concentration of 0.5 - 1 µg/ml. |
Target
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Relevance
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. -
Cellular localization
Nuclear -
Alternative names
- c-myc tag antibody
- Myc Epitope Tag antibody
Images
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Western blot - Biotin Anti-Myc tag antibody (ab34773)This image is courtesy of an anonymous AbreviewMouse NIH 3T3 cells over-expressing pGEN IRES pyk2 Myc-tag. ab34773 was used at 1/2500 dilution. Please see accompanying abreview for additional information.
Protocols
Datasheets and documents
References (2)
ab34773 has been referenced in 2 publications.
- Chakraborty M et al. Microtubule end conversion mediated by motors and diffusing proteins with no intrinsic microtubule end-binding activity. Nat Commun 10:1673 (2019). PubMed: 30975984
- Hayes HL et al. Pdx-1 activates islet a- and ß-cell proliferation via a mechanism regulated by transient receptor potential cation channels 3 and 6 and extracellular signal-regulated kinases 1 and 2. Mol Cell Biol 33:4017-29 (2013). PubMed: 23938296
